摘要
目的探讨β3肾上腺素能受体(β3-AR)基因T190C(Trp64Arg)遗传多态性与原发性高血压病(EH)患者首次心脑血管事件的相关性。方法获取我国汉族正常血压组306例和EH组372例外周静脉血,提取DNA,应用聚合酶链式反应-限制性片段长度多态性分析、琼脂糖凝胶电泳等技术检测β3-AR T190C突变基因型;所有研究对象均收集年龄、性别、体重指数(BMI)、肌酐、尿酸、甘油三酯(TG)、空腹血糖等生化指标,并记录EH患者高血压病程及首次心脑血管事件发生时间;统计分析采用t检验、方差分析、协方差、卡方检验和Kap-lan-Meier分析。结果经Hardy-Weinberg遗传平衡检验,β3-ART190C突变基因型分布频率符合Hardy-Weinberg遗传平衡(P均>0.05),说明样本具有代表性;β3-ART190C等位基因和基因型的分布频率在两组间的差异无统计学意义;两组合并后CC突变基因型的BMI和TG明显高于TT基因型,BMI分别为25.34±2.81和24.16±3.08(kg/m2,P<0.05),TG分别为2.46±1.08和1.66±1.07(mmol/L,P<0.05),调整混杂因素后这种关系依然存在;心脑血管事件组与无心脑血管事件组EH患者血压间的差异无统计学意义,回顾性Kaplan-Meier分析(Breslow模型)显示:携带C突变等位基因(CC纯合子和CT杂合子)EH患者15年内发生首次心脑血管事件风险较TT纯合子高(P<0.05)。结论β3-AR 190T→C基因变异可能是中国汉族人群TG增高和肥胖发病的一种危险因素,携带C突变等位基因的EH患者发生心脑血管事件风险增加。
Objective To investigate the association between polymorphisms of β3 adrenergic receptor （β3-AR） gene and the first cardiovascular and cerebrovascular events （CVEs） in hypertensive patients. Methods β3-AR gene T190C polymorphisms were detected with polymerase chain reaction-restrict fragment length polymorphism （PCR-RFLP） method in 372 patients with essential hypertension and 306 normotensive subjects. Basic clinical dates such as age, sex, body mass index （ BMI ） , creatinine, uric acid, triglyceride （TG）, fasting plasma glucose, hypertensive history and the first CVEs in hypertensive patients were collected. T Test, Analysis of Variance, Covariance, Chi-square Test and Kaplan-Meier were selected. Results The frequencies of T190C genotypes of β3-AR gene were in agreement with Hardy-Weinberg equilibrium in our study. The frequencies of T190C allele and genotypes were no significant difference in the two groups （P 〉 0. 05 ）. The BMI and TG level in all subjects with CC genotype were significantly higher than that in all subjects with Tr genotypes. BMI was 25.34 ± 2. 81 and 24. 16 ± 3.08 （ kg/m^2, P 〈 0.05 ）, respectively. TG was 2. 46 ±1.08 and 1.66 ± 1.07 （ mmot/L, P 〈 0. 05 ）, respectively. The relationship remains after adjusted other risk factors. The blood pressure was not significantly difference between hypertensive patients with CVES and those without CVEs. The CVEs in hypertensive patients with C allele were earlier than TT genotype （ P 〈 0. 05）. Conclusions 190T→C genovariation of β3-AR gene might be a risk factor in increase of TG level and BMI in Chinese Han people. Hypertensive patients with C allele might increase the risk of CVEs.
出处
《中华临床医师杂志（电子版）》
CAS
2008年第12期36-40,共5页
Chinese Journal of Clinicians(Electronic Edition)
基金
福建省自然科学基金重点资助项目(C0420003)
关键词
高血压
受体
肾上腺素能Β3
多态性
限制性片段长度
心脑血管事件风险
Hypertension
Receptors, adrenergic, beta-3
Polymorphisms, restriction fragment lergth
Cardiovascular and cerebrovascular events risk
