摘要
目的:克隆并分析保加利亚德氏乳杆菌中的乳糖酶基因。方法:利用PCR技术从保加利亚德氏乳杆菌中克隆出乳糖酶基因、测序并生物信息学分析。结果:成功的从保加利亚德氏乳杆菌中克隆出全长为3024bp的乳糖酶基因,利用生物软件分析,推测乳糖酶基因共编码1008个氨基酸,蛋白分子量为114KDa,等电点为4.9,氨基酸序列中共有9处潜在的糖基化位点。并将此基因与不同来源的乳糖酶基因进行同源性比较。结论:成功的克隆出乳糖酶基因,并利用生物分析软件对其进行生物信息学分析。了解该酶的性质特征,为进一步研究及低成本表达该酶奠定基础。
Objective： To clone and analyze lactase gene from Lactobacillus delbrueckii bulgaricus, Methods： Cloned lactase gene from Lactobacillus delbrueckii bulgaricus with PCR, made sequencing and bioinformatics analysis. Results： Cloned lactase gene （3 024 bp） successfully. It was presumed that the lactase gene encode 1 008 amino acids, with protein molecule 114 KDa, isoelectric point 4.9, 9 potential glycosylation sites in amino acid sequence. Made homology comparison with other lacteses. Conclusion： The lactase gene is cloned successfully and the bioinformatics analysis is made by biological analysis software to investigate its character. It provides foundation for further study and colonization at low cost.
出处
《海南医学院学报》
CAS
2009年第2期104-106,132,共4页
Journal of Hainan Medical University
基金
海南医学院重点学科项目(海医科研部[2005-46])
