摘要
【目的】研究S100蛋白在动情周期SD大鼠子宫中的分布情况及其变化规律。【方法】采用阴道涂片方法将16只雌性SD大鼠分为动情前期、动情期、动情后期和动情间期4组,处死各组大鼠取子宫制作石蜡切片,采用免疫组织化学超敏感法(Streptavidin-Peroxidase,SP),研究S100蛋白在大鼠动情周期子宫中的分布规律。【结果】S100蛋白免疫阳性产物在各期子宫各层中均有不同程度的分布,主要定位于子宫内膜上皮细胞、腺上皮细胞、基质细胞、血管内皮细胞、血管平滑肌细胞、肥大细胞、平滑肌细胞及成纤维细胞中。S100蛋白免疫阳性产物在动情周期子宫内膜、肌层和外膜中的表达变化规律一致:动情前期着色最弱,动情期着色最深,动情后期着色变浅,动情间期着色又加深,表达呈双峰变化趋势。【结论】S100蛋白在动情周期SD大鼠子宫中的表达具有一定规律,可能受性类固醇激素的调节。
[Objective] The trail was conducted to study the expression of S100 protein in uterus of the rats during estrus cycle. [Method] Sixteen mature female SD rats were divided randomly into 4 groups： preoestrus, estrum, metoestrus and diestrus, by vaginal smear, the expression of S100 protein in uterus of the rats during estrus cycle was studied by immunohistochemical SP method. [Result] The expression and variation of S100 protein exist in uterus in rat estrous cycle. The immunoreactive products are mainly located in endometrial epithelial cells, epithelial cells, stromal cells, endothelial cells, vascular smooth muscle cells, mast cells, smooth muscle cells and fibroblasts. There is no much difference in the expression regulation of S100 protein immunoreactive products in muscular and the endometrium in different periods, but there are some similar rules to follow： the expression of S100 protein in proestrus is slight, then it is strongly expressed in estrus,while it decreases in metoestrus. At last,the expression of S100 is re-increased in metestrus. They present the bimodal distribution. [Conclusion]The results show that the expression of S100 protein in the uterus follows some certain rules and might be mediated by the sex steroid hormone.
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2009年第1期17-21,28,共6页
Journal of Northwest A&F University(Natural Science Edition)
基金
国家自然科学基金项目(39670550)
西北农林科技大学科研专项(06ZR003)
