摘要
【目的】分析灰斑古毒蛾核型多角体病毒（Orgyia ericae nucleopolyhedrovirus，OrerNPV）EcoRⅠ-U片段分子生物学特征，为研究其分子特性及其在OrerNPV感染周期中的功能提供科学依据。【方法】克隆、分析OrerNPV EcoRⅠ-U片段所包含的若干基因，并应用生物软件对OrerNPV泛素基因（Ubi基因）进行了进化分析。【结果】OrerNPV EcoRⅠ-U片段包含Ubi基因、AcORF34同源基因和39K基因部分序列，在该区域中编码Ubi基因的开放阅读框（ORF）由246个核苷酸组成，可以编码81个氨基酸，预汁蛋白质分子质量为8．9ku。OrerNPV Ubi基因与苜蓿丫纹夜蛾核型多角体病毒（AcMNPV）、家蚕核型多角体病毒（BmNPV）、薄荷灰夜蛾核型多角体病毒（RaouM-NPV）的同源性最高，均达92％，与小菜蛾颗粒体病毒（Plutella xylostella granulovirus，PxGV）的同源性最低，为62％。OrerNPV与AcMNPV、BmNPV和RaouMNPV的亲源关系最近。EcoR Ⅰ-U片段＋M13端含有编码39K基因（pp31）的部分序列，该多肽与10种NPV的39K序列有48％～52％的同源性。【结论】明确了OrerNPV EcoRⅠ-U片段的序列特征及OrerNPV Ubi在泛素蛋白家族中的进化关系。
[Objective] Molecular characteristics were analyzed on Orgyia ericae nueleopolyhedrovirus （OrerNPV）. [Method] Here, the cloned OrerNPV EcoRⅠ-U fragment in which the Ubi and homologues of AcORF34 and 39K were found was analyzed. [Result] The Ubi open reading frame was 246 nucleotides long that coded a peptide of 81 amino acids with a predicted molecular weight of 8.9 ku. The peptide of OrerNPV Ubi shared a highest similarity of 92% to Autographa ealifornica multiple nucleocapsid nucleopolyhedrovirus,Bornbyx mori nucleopolyhedrovirus and Rachiplusia ou multiple nucleocapsid nucleopolyhedrovirus;while it shared a lowest similarity with Plutella：rylostella granulovirus GV of 62%. The phylogenetic results of Ubi based on 21 species showed that AcMNPV,BmNPV and RaouMNPV were mostly related to OrerNPV. The 39K amino acid residues in the M13＋ terminal of OrerNPV had 48%-52% identities with homologous genes from other 10 NPVs. [Conclusion] The result clarified the characteristics of OrerNPV EcoR Ⅰ-U fragment and phylogenetic relationships of OrerNPV Ubi gene in the Ubi family.
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2009年第1期155-160,共6页
Journal of Northwest A&F University(Natural Science Edition)
基金
河南省基础与前沿项目(072300430080)
河南省农业科学院博士启动项目
