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猪胸膜肺炎放线杆菌APXⅠ A蛋白的截断表达与鉴定 被引量：3

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摘要 溶血外毒素(Apx)是胸膜肺炎放线杆菌(APP)最主要的毒力因子及保护性抗原。利用PCR技术扩增APP血清5型编码APXⅠN-端具有保护性抗原表位的APXⅠA部分基因,扩增片段长度为1 149 bp。将其插入原核表达载体PET-32a中,转化至E.coilBL21中诱导表达。SDS-PAGE分析结果表明APXⅠA基因能在大肠杆菌中高效表达,融合蛋白的分子量约为40 ku。Western-blot结果表明该融合蛋白能被APP阳性猪血清所识别,指示表达的蛋白具有良好的免疫活性,为建立猪传染性胸膜肺炎的诊治及高效亚单位疫苗的研制奠定了基础。
作者 徐公义 王海丽 葛长城 吕云鹏 吕俊
机构地区 聊城职业技术学院 中国农业大学
出处 《畜牧与兽医》 北大核心 2008年第10期67-69,共3页 Animal Husbandry & Veterinary Medicine
关键词 胸膜肺炎放线杆菌 APX I A 表达
分类号 S858.28 [农业科学—临床兽医学]
  • 相关文献

参考文献12

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二级参考文献15

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共引文献41

同被引文献22

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引证文献3

二级引证文献7

畜牧与兽医
2008年 第10期

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