摘要
利用D50/HB277衍生的由190个株系组成的F7重组自交系群体,在杭州和海南两种环境条件下对剑叶形态及稻米粒形进行QTL分析。共检测到控制上述性状29个QTL,分布于除第2和第11染色体以外的其它所有染色体,其中6个QTL在两种环境中同时被检测到且贡献率大于10%。第1、4和7染色体分别存在控制剑叶面积、剑叶宽度及稻米粒形等性状且贡献率较大的QTL区间,同时在上述区间还存在控制其它多个性状,表现为QTL成簇分布,并以此筛选了3个剩余杂合体,作为相关基因遗传分解、精细定位、克隆及分子设计育种的候选区域。
A F7 recombinant inbred population consisting of 190 lines, derived from cross of ajavonica （D50） and an indica （HB277） rice was used for QTL analysis for flag leaf morphological traits and grain shape in Hangzhou and Hainan. A total of 29 QTLs for flag leaf and grain shape were detected on 12 chromosomes except chromosome 2 and chromosome 11, and 6 QTLs were detected in two different growing environments and the contribution of these QTLs are more than 10%. Main-effect QTLs for flag leaf area,flag leaf width and grain shape were detected on chromosome 1.4 and 7, respectively. At the same time, these regions were also with QTLs for other traits, and they acted as QTL clusters, so three residual heterozygous lines of above region were selected for candidate region as gene genetic dissection、 cloning and molecular design breeding.
出处
《分子植物育种》
CAS
CSCD
2009年第1期16-22,共7页
Molecular Plant Breeding
基金
国家高技术研究发展计划项目(2006AA100101)
科技部国际合作项目(2006DFA32130)资助
