摘要
目的探讨基质金属蛋白酶（matrix metalloproteinase，MMP）2和9在不同部位的中耳胆脂瘤的表达差异及其骨破坏作用。方法采用免疫组化方法检测上鼓室胆脂瘤18例、鼓室窦胆脂瘤16例、胆脂瘤患者外耳道皮肤10例、正常外耳道皮肤10例中MMP-2和MMP-9的表达。结果MMP-2、MMP-9在上鼓室胆脂瘤和鼓室窦胆脂瘤上皮各层细胞中均有表达，基底层表达最强；鼓室窦胆脂瘤较上鼓室胆脂瘤染色深。正常外耳道皮肤和胆脂瘤患者外耳道皮肤有非常浅的表达，主要分布在基底层细胞的胞质中。MMP-2、MMP-9在正常外耳道皮肤和胆脂瘤患者外耳道皮肤的表达差异无统计学意义（P值均〈0．05）；上鼓室胆脂瘤和鼓室窦胆脂瘤中MMP-2、MMP-9的表达均高于正常外耳道皮肤和胆脂瘤患者外耳道皮肤，差异有统计学意义（P值均〈0．05）；鼓室窦胆脂瘤中MMP-2、MMP-9的表达高于上鼓室胆脂瘤，差异具有统计学意义（P值均〉0．05）。胆脂瘤上皮中MMP-2和MMP-9的表达呈显著正相关（r=0．974，P〈0．01），MMP-2的表达高于MMP-9。MMP-2和MMP-9的表达与听小骨的破坏程度呈正相关（r=0．789及0．803，P值均〈0．01）。结论胆脂瘤型中耳炎骨质破坏过程中MMP起重要作用。鼓室窦胆脂瘤的骨质破坏程度较上鼓室胆脂瘤重，更具侵袭性，更容易产生颅内外并发症。
Objective To study various expressions and roles of MMP-2,9 in cholesteatoma from different positions. Methods MMP-2 and MMP-9 were detected with immunohistochemiscal methods using antibody in 18 attic cholesteatoma, 16 tympanic sinus cholesteatoma, 10 auditory meatal skin in cholesteatoma（CAMS） and 10 normal auditory meatal skin （NAMS）. Results The stain in cholesteatoma was stronger than that in CAMS and NAMS （P 〈0. 05）. Expression of MMP-2,9 was seen in all layers of the epithelium in cholesteatoma and the stain was strongest in basement membrane. Expression of MMP-2,9 in tympanic sinus cholesteatoma was stronger than that in attic cholesteatoma. The expression of MMP-2 and MMP-9 were closely related in cholesteatoma （ r = 0. 974, P 〈 0. 01 ）. The expression of MMP-2,9 in cholesteatoma was related with destruction of ossicular chain（ r = 0. 789, P 〈 0. 01 ; r = 0. 803, P 〈 0.01 ）. Conclusions MMPs play a very important role in bone destruction in cholesteatoma. The destruction of bone in tympanic sinus cholesteatoma is more severe than that in attic cholesteatoma. Tympanic sinus cholesteatomas are more invasive in destruction surround bone matrix.
出处
《中华耳鼻咽喉头颈外科杂志》
CAS
CSCD
北大核心
2009年第1期44-48,共5页
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
关键词
基质金属蛋白酶2
基质金属蛋白酶9
胆脂瘤
中耳
骨质吸收
Matrix metalloproteinase 2
Matrix metalloproteinase 9
Cholesteatoma, middle ear
Bone resorption
