摘要
为了探讨烟用香精香料对人支气管上皮细胞的毒性作用,并为其安全性评价提供毒理学依据,将永生化的人支气管上皮细胞(BEP2D细胞)分为纯卷烟组和卷烟加香加料组、卷烟加香精和卷烟加香料组、卷烟加不同香精组、卷烟加不同香料组,用MTT法测定细胞存活率,用分子探针氢化乙锭(HE)的荧光产物溴乙锭(EB)相对定量细胞内活性氧(ROS)水平。结果表明加香加料致卷烟烟气冷凝物(CSC)的细胞存活率降低,细胞内ROS水平增高,对细胞的氧化损伤大;与香料比较,香精的细胞存活率低,对细胞的氧化损伤大;1#香精的细胞存活率较高,但对细胞的氧化损伤较大;1#香料的细胞存活率较低,但对细胞的氧化损伤较小,表现在细胞内ROS水平较低。由此可知CSC对BEP2D细胞有毒性,烟用香精香料的添加增加了烟气对BEP2D细胞的毒性作用,而在香精和香料中,香精对细胞毒性较大。
The toxic effects of tobacco essence and flavor on human bronchial epithelial cells were studied to provide toxicological basis for the safety evaluation. Exponentially growing immortalized human bronchial epithelial （BEP2D） cells were used. The experiments were carried out contained of （1） the pure tobacco group and tobacco essence and flavor group, （2） tobacco essence group and tobacco flavor group, （3） different tobacco essence group, （4） different tobacco flavor group. The cell survival rates were measured by MTT method. Ethidium bromide, fluorescent products of the molecular probes-hydroethine, was used to monitor the intracellular reactive oxygen species （ROS）. The results showed that in the group added tobacco essence and flavor, the BEP2D cells survival rates decreased, and the level of ROS productions in BEP2D cells increased for its high oxidative damage. Comparison of the tobacco essence with tobacco flavor showed that the essence induced low survival rates and high oxidative damage to BEP2D cells. In the group added 1# essence, the cell survival rates increased with high oxidative damage. In the group added 1# flavor, the BEP2D cells survival rates decreased with low oxidative damage, because the level of ROS productions in BEP2D cells also decreased. It implied that cigarette smoke condenses had toxicity to BEP2D cells, and the tobacco essence and flavor enhanced the toxicity of BEP2D cells; What＇s more, tobacco essence had higher cytotoxicity than tobacco flavor.
出处
《湖南农业科学》
2009年第1期80-84,共5页
Hunan Agricultural Sciences
关键词
烟用香精香料
人支气管上皮细胞
细胞存活率
活性氧
卷烟烟气冷凝物
细胞毒性
tobacco essence and flavor
, human bronchial epithelial cells （BEP2D cells）
cell survival rate
reactive oxygen species （ROS）
cigarette smoke condense
cytotoxicity
