摘要
以ＮＡＤＨ诱导的心肌线粒体损伤体系为模型，在体外用ＥＳＲ研究了谷胱甘肽（ＧＳＨ）、超氧化物歧化酶（ＳＯＤ）及辣根过氧化物酶（ＨＲＰ）的抗氧化机制．结果表明，低浓度的ＧＳＨ可部分抑制体系中自由基的生成；ＳＯＤ与ＨＲＰ以适当比例共同作用，可完全抑制体系中自由基的生成，较好地防止脂质过氧化的发生．
Experiments were carried out using ESR spectra with 5,5 dimethyl 1 pyrroline N oxide (DMPO) as spin trap to study the antioxidant mechanism of glutathione (GSH), superoxide dismutase (SOD) and horseradish peroxidase (HRP). NADH induced lipid peroxidation system was taken as an in vitro model. The results indicated that GSH could partly inhibit the generation of free radicals at low concentration, while SOD and HRP at proper ration could completely scavenge free radicals, thus prevent mitochondria lipid peroxidation from the damage of lipid peroxidation.
出处
《波谱学杂志》
CAS
CSCD
北大核心
1998年第2期139-143,共5页
Chinese Journal of Magnetic Resonance
基金
吉林大学酶工程实验室开放课题资助
