摘要
S-层蛋白(surface layer protein,slp)是一些乳酸菌,细菌及古细菌细胞壁表面所包被的生物活性大分子。这些蛋白能够形成周期性的有序结构,如倾斜形,正方形或是匀称的六边形。最新研究表明,这些结构是通过非共价键相互作用,在熵驱动下自我组装而成。与其他S-层蛋白相比,乳酸菌S-层蛋白的显著特点是分子质量小、等电点高。一些乳酸菌含有多种S-层蛋白编码基因,他们能够分别或同时表达。随后,文中介绍了3种乳酸菌S-层蛋白的分离方法。最后简单介绍了乳酸菌S-层蛋白在益生菌微生态制剂中的的应用。
Surface layers that are composed of crystalline arrays of proteinaceous subunits present as the outermost component of cell wall in several species of the genus Lactobacillus, as well as in many other bacteria and Archaea. These proteins form a periodic ordered structure with oblique, square or hexagonal type symmetry. It is currently believed that these structures are formed due to entropy-driven self-assembly of the protein subunits, which are attached by non-covalent interactions. The typical characteristics that distinguish Lactobacillus S-layer proteins from other surface layer proteins are small size and predictable high pI value. Several lactobacilli possess multiple surface layer protein genes can be differentially or simultaneously expressed. Three methods for the isolation of Lactobacillus surface layer proteins were discussed later. The use of Lactobacillus surface layer proteins in probioties microeeological agent was also discussed.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2008年第11期124-130,共7页
Food and Fermentation Industries
基金
国家863项目资助(项目编号:2007AA10Z347)
