摘要
目的:研究西红花酸对大鼠酒精性脂肪肝的改善作用,探讨其可能的作用机制。方法:SD大鼠ig酒精10周,建立大鼠酒精性脂肪肝模型,同时给予西红花酸(50 mg.kg-1,ig,qd)进行预防治疗,测定血清丙氨酸氨基转移酶(ALT),血清和肝脏三酰甘油(TG)水平,肝脏中游离脂肪酸(FFA)含量,极低密度脂蛋白(VLDL)分泌速率,线粒体和过氧化物酶体脂肪酸β-氧化速度,苯胺羟化酶(ANH),乙醇脱氢酶(ADH),乙醛脱氢酶(ALDH)活性等生化指标并进行病理组织学观察。结果:西红花酸能够降低血清ALT和TG水平,升高脂肪酸线粒体β-氧化能力,降低肝脏TG和FFA水平,此外西红花酸还可使微粒体ANH活性降低,使胞浆中ADH和ALDH活性升高,但对VLDL分泌速率和过氧化物酶体β-氧化没有影响。结论:西红花酸对酒精性脂肪肝的作用机制与加速肝脏线粒体脂肪酸氧化,减轻肝脏脂肪堆积,加速乙醇和乙醛的清除,调控酒精代谢过程,提高机体抗氧化能力有关。
Objective： To investigate the improving effect of crocetin in rats with alcoholic fatty liver, and to elucidate the possible mechanism. Methods： Alcoholic fatty liver in rats were induced by feeding high lipid diet plus alchol for 10 weeks. Crocetin （50 mg·kg^-1, ig, qd） was administered throughout the experiment. Then, liver index, TG, FFA, rate of VLDL-secretion, activity of aniline hydroxylase （ANH） , alcohol dehydrogenase （ADH） , aldenhyde dehydrogenase （ALDH） were measured. Alanine aminotransferase （ALT） activity and TG concentration in serum and pathological changes in liver were also observed. Results： Crocetin decreased liver index, serum ALT activity and TG level, increased mitochondrial β-oxidation, and decreased the levels of liver TG and FFA. However, crocetin did not affect VLDL-secretion and peroxisomal β-oxidation. Meanwhile, crocetin decreased the activity of ANH and raised the activities of ADH and ALDH. Conclusion： Crocetin can protect rats against alcholic fatty liver. This effect relates to the increase of mitochondrial-β-oxidation, reduction of fatty sediment, inhibition of lipid peroxidation, and acceleration of the elimination of alcohol and aldehyde.
出处
《中国新药杂志》
CAS
CSCD
北大核心
2008年第24期2115-2118,共4页
Chinese Journal of New Drugs
关键词
酒精性脂肪肝
西红花酸
代谢
脂质过氧化
alcoholic fatty liver
crocetin
metabolism
lipid peroxidation
