摘要
用肌肉注射HCG的处理方式（剂量为500U／kg体重，每周注射1次，注射时间为6周）诱导雄性花鳗鲡性腺发育成熟，成熟率达80．0％。对人工催熟花鳗鲡精子的生物学特性研究结果表明：花鳗鲡精子头部长径为（3．81±0．69）μm，短径为（1．24±0．15）μm；尾部长度为（24．83±3．05）μm；精液pH为7．3～7．5，精子密度每毫升1．02×10^10尾。精子的适宜盐度为15～20，其中盐度为15时，精子激活比率最高，快速运动时间以及精子的寿命最长。精子的pH适宜范围为6．0～8．0，pH值过高或过低都会影响精子的活力与寿命。另外，4种金属离子（Mg^2＋、Ca^2＋、Na^＋和K^＋）对花鳗鲡精子活力与寿命的影响趋势基本一致，金属离子浓度过高或过低都会抑制精子的活力、缩短快速运动时间和寿命。而MgCl2、CaCl2、KCl、NaCl溶液浓度为0．4～0．6g／mL时，精子活力最好，最高激活比率为3级（41．0％～60．0％）。
In this study, artificial induction of testicular development and maturation in male marble eels was carried out by muscular injection of HCG （ 6 weekly injection of HCG at a dose of 500 U/kg per body weight） and the rate of sexual maturation reached 80.0%. The long diameter and tile short diameter of the spermic head was （3.81 ±0.69）μm, and （1.24 ±0.15）μm, respectively. The length of the spermic tail was （24.83 ±3.05） μm. The pH value of sperm was 7.3 - 7.5. The density of spermatozoa from marble eels was 1.02 × 10^10 ind/mL. The range of suitable salinity was 15 - 20, when the salinity of water was 15, the spermatozoa had the best motility, and the spermatozoa had the longest life. The suitable pH of spermatozoa was 6.0 - 8.0, high or low pH value could restrain the mobility and shortened the longevity. The trends that affected the spermatozoa mobility and life span were almost consistent by four kinds of metallic ions （Mg^2＋ , Ca^2＋ , Na^＋ , and K^＋ ）. When the concentration of solution was too low or too high, the spermatozoa mobility was restrained, and the spermatozoa quick movement time and life span were shortened. When the concentration of MgCl2, CaCl2, KCl, and NaCl solutions were 0.4 - 0.6 g/mL, the spermatozoa mobility was the best, and the highest initiated rate of the spermatozoa reached 41.0% - 60.0%.
出处
《水产学报》
CAS
CSCD
北大核心
2009年第1期53-59,共7页
Journal of Fisheries of China
基金
国家自然基金面上项目(30770283)
“十一五”国家科技支撑计划重点项目(2007BAD29B03)
海南省教育厅高等学校科学研究项目(Hjkj2008-22)
广东省科技兴海(渔)项目(B200701A06)
关键词
花鳗鲡
人工诱导
精巢
精子活力
Anguilla marmorata
artificial induction
testis
spermatozoa mobility
