摘要
目的探讨中药治疗寻常型银屑病的机制。方法同期收治的寻常型银屑病患者30例，中医辨证分型血热症10例（观察1组）、血瘀症10例（观察2组）、血燥症10例（观察3组）。均取外周血体外诱导培养树突状细胞（DC），成熟后分为2份，一份为空白对照组；另一份接观察1组、2组、3组分别加入凉血活血汤、活血散瘀汤、养血解毒汤，剂量为30ml／L。采用ELISA法测定上清中白细胞介素-12（IL-12）、白细胞介素-4（IL-4）、γ-干扰素（IFN-γ）和白细胞介素-1β（IL-1β）水平。结果与空白对照组比较，观察1—3组IL-12、IFN-γ水平明显降低，IL-1β水平明显升高，观察2组、3组IL-4水平亦明显升高。结论中药可通过改变DC细胞因子水平调节机体Th1／Th2平衡，此为其治疗寻常型银屑病机制之一。
Objective To investigate the adjustive effect of compound traditional Chinese medicine on the cytokines of dendritic cells （DC） in peripheral blood from patients with psoriasis vulgaris in vitro. Methods DC in peripheral blood from patients with psoriasis vulgaris were cultured for five days with medium which contained GM-CSF and IL-4, then divided into the observed groups and the contral group. The compound traditional Chinese medicine were added into the observed groups and the cells were collected on the 5th day. The levels of IL-12,IFN-γ,IL-4 and IL-1β in cell culture supernatant were detected by ELISA. Results Compared with the control group, the level of IL-12 and IFN-γin the observed groups decreased obviously, and the level of IL-1β and IL-4 increased obviously. Conclusion The compound traditional Chinese medicine can regulate the cytokines of DC,which maybe the mechanism of it to treat the psoriasis vulgaris.
出处
《山东医药》
CAS
北大核心
2008年第44期13-14,共2页
Shandong Medical Journal
基金
石家庄市科技攻关计划资助项目(071461143)
