摘要
目的检测骨髓基质细胞条件培养液(N-CM)中诱导神经干细胞(NSCs)向神经元方向分化的细胞因子,探讨骨髓基质细胞(BMSCs)调节NSCs分化的机制。方法将收集到的N-CM混匀后经超滤浓缩分为大于5kD和小于5kD两部分,用这两部分培养液分别培养NSCs,确定其中能促进NSCs分化为高比例神经元的部分并进行大鼠蛋白质微阵列检测。结果N-CM分子量大于5kD的部分可以促进NSCs分化为高比例神经元,并通过蛋白质微阵列检测,与单纯骨髓基质培养液相比,有7种细胞因子的表达量上调了1.5倍,分别是CINC-3、CNTF、IFN-γ、IL-1α、MCP-1、TIMP-1和VEGF。结论BMSCs分泌的可溶性分子对NSCs分化为神经元有促进作用,其中某些分子量大于5KD的细胞因子可能在这一调节作用中发挥效应。
Objective To detect the cytokines in the bone marrow stromal cells （BMSCs） conditioned medium （neurobasal conditioned medium ,N-CM） that can regulate the differentiation of neural stem cells （NSCs） into high proportional neurons and explore the mechanism that BMSCs regulate the differentiation of NSCs. Methods The collected N-CM was divided into two parts（ 〉 5kD and 〈 5kD）by means of ultrafiltration after misce bene. The two parts were used to culture NSCs separately, and the effective part that could regulate the differentiation of NSCs was detected by protein mieroarray analysis. Results The N-CM 〉 5kD could promote the NSCs to differentiate into high proportional neurons, so this part was detected by protein microarray analysis, 7 cytokines CINC-3, CNTF, IFN-γ, IL-1α, MCP-1, TIMP-land VEGF were detected to up-regulate 1.5 times compared with the neurobasal medium （molecular weight above 5kD）. Conclusion The dissolvable molecules excreted by BMSCs could promote the NSCs to differentiate into neurons. Some cytokines with the molecular weight of above 5kD play a crucial role during this process.
出处
《解剖学报》
CAS
CSCD
北大核心
2008年第6期845-849,共5页
Acta Anatomica Sinica
基金
国家自然科学基金资助项目(30400138)
河北省自然科学基金资助项目(2008000993)
关键词
神经干细胞
骨髓基质细胞
条件培养液
蛋白质微阵列
细胞因子
超滤浓缩
免疫组织化学
Neural stem cell
Bone marrow stromal cell
Conditioned medium
Protein microarray
Cytokine
Uhrafiltrate concentration
Immunohistochemistry
