摘要
目的：研究２Ｈｚ及１００Ｈｚ电针耐受对κ受体ｍＲＮＡ转录的影响。方法：每天给大鼠２Ｈｚ及１００Ｈｚ电针１次，每次３０ｍｉｎ，连续电针６ｄ，在电针的第１、３、６天分别取各个脑区及脊髓，提取ＲＮＡ，然后用反转录聚合酶链反应（ＲＴＰＣＲ）方法检测κ受体ｍＲＮＡ水平。结果：（１）电针后的第１天，各脑区及脊髓κ受体ｍＲＮＡ均无明显改变；（２）每天给大鼠针刺（不给电刺激）１次，连续多次针刺可使纹状体内κ受体ｍＲＮＡ显著增加，在针刺的第６天杏仁核内κ受体ｍＲＮＡ也轻度增加；（３）每天给大鼠１００Ｈｚ电针１次，每次３０ｍｉｎ，连续电针３～６次可使脊髓中κ受体ｍＲＮＡ水平下降，同样现象也可在纹状体及杏仁核中观察到，２Ｈｚ电针的结果很不一致，它使ＰＡＧ和纹状体内κ受体ｍＲＮＡ水平降低，而杏仁核和脊髓中的κ受体水平增强，或呈双相反应。结论：单纯针刺３～６ｄ本身有一定的中枢效应，但是多次电针则在大多数情况下抑制κ受体ｍＲＮＡ的转录，提示内源性阿片肽大量释放也可引起阿片受体下调，这可能是电针耐受的机制之一。
Objective: To observe the effects of 2 Hz and 100 Hz electroacupuncture(EA) stimulation on the transcription of CNS κ opioid receptor mRNA. Methods: EA in 2 Hz or 100 Hz was administered 30 min once a day for 6 days, total RNA extracted from discrete brain regions and the spinal cord on the first, third, sixth days and the level of mRNA detected with the method of reversetranscriptionpolymerase chain reaction(RTPCR). Results: (1) No significant change of the abundance of κ receptor mRNA was observed in various brain areas and spinal cord 24 h after one session of EA stimulation. (2)In rats receiving acupuncture without electricstimulation once (30 min) every day for 6 days, the mRNA level of κ opioid receptor increased significantly in the striatum, as well as in amygdala on the 6th day. (3)A significant decrease of the κ receptor mRNA level was found after 3～6 sessions of 100 Hz EA stimulation in the spinal cord, striatum and amygdala. In contrast, changes induced by repeated 2 Hz EA stimulation were not consistent, κ receptor mRNA level decreased in PAG and striatum, increased or changed biphasically in the amygdala and spinal cord. Conclusion: (1) Insertion of the acupuncture needle in the acupiont without giving eletric stimulation might produce certain CNS effects. (2)In most cases, repeated EA stimulations inhibited the transcription of κ opioid receptor t hat may lead to the downregulation of κopioid receptor. This may serve as one of the molecular mechanisms for the development of EA tolerance.
出处
《北京医科大学学报》
CSCD
1998年第1期1-4,共4页
Journal of Peking University(Health Sciences)
基金
国家自然科学基金
关键词
电针
针灸耐受
受体
阿片样
阿片受体
MRNA
Electroacupuncture Acup mox tolerance Receptors, opioid, kappa RNA, messenger/metab Doseresponse relat, acup mox
