摘要
目的:观察P14ARF、E2F1蛋白在结直肠肿瘤的表达,明确其对肿瘤发生及侵袭发展的影响。方法:应用免疫组化方法检测尸检正常结直肠组织6例,结肠腺瘤30例,不伴淋巴结转移(无LNM)结直肠癌35例,伴有淋巴结转移(有LNM)结直肠癌30例,后者再分为手术切缘组织、结直肠癌组织、淋巴结转移癌组织,共6组的P14ARF、E2F1蛋白表达情况,采用半定量和图像分析定量测定含量。结果:①结直肠正常黏膜组织、腺瘤组织、癌组织P14ARF、E2F1的表达阳性率逐渐减少,差异存在统计学意义(P<0.05)。②P14ARF、E2F1在伴有淋巴结转移、肝转移的癌组织内表达阳性率与不伴转移的癌组织之间差异无统计学意义(P>0.05)。③P14ARF、E2F1蛋白表达在不同临床病理特征的关系之间差异无统计学意义(P>0.05)。结论:①P14ARF、E2F1具有抑制癌发生作用,蛋白异常仅是结直肠癌癌变早期事件,与淋巴结、远处器官转移等侵袭活动无关,与临床病理特征无关。②P14ARF、E2F1相互协同抑制细胞生长和诱导凋亡,因而可以作为治疗结直肠癌的新靶点。
Objective：To investigate the expressions of P14ARF and E2F1 in human colorectal tumor and their influence on the tumorigenesis and development of the disease. Methods ： The expressions of P14ARF and E2F1 were detected at the protein level by immunohistochemieal test in 17 samples of autopsic normal coloreetal mucosa tissues, 30 samples of colorectal adenoma tissues, 35 samples of colorectal cancer tissues without lymphonode metastasis and 32 with lymphonode metastasis, the latter including surgically resected marginal normal mucosa tissues, colorectal cancer tissues and lymphonode metastasis tissues. The positive signals were analyzed by semi quantitative and HPIAS-1000 quantitative method. Results： The positive expression rates of P14ARF and E2F1 decreased successively from colorectal normal mucosa tissues to adenoma tissues and cancer tissues, with statistically significant differences among the 3 groups （ P 〈 0.05 ） , but not between the cancer tissues with lymphonode and liver metastases and those without （P 〉 0.05 ） , nor were there obvious differences in the expressions of P14ARF and E2F1 with different clinicopathologic characteristics （ P 〉 0. 05 ）. Conclusion ： P14ARF and E2F1 can suppress colorectal tumorigenesis. The protein dysfunction is merely an early event in the tumorigenic process, correlated neither with such invasive activities as lymphonode and distant organ metastases nor with clinicopathologic characteristics. P14ARF and E2F1 have a synergenic action in suppressing cell growth and inducing cell apoptosis, and therefore can be used as potentially active gene therapeutic agents for coloreetal cancer.
出处
《医学研究生学报》
CAS
2009年第1期16-19,23,114,共6页
Journal of Medical Postgraduates
基金
云南省教育厅科学研究基金项目(项目编号:5Z0506C)
