摘要
微小按蚊（Ａｎｏｐｈｅｌｅｓｍｉｎｉｍｕｓ）是东南亚及我国南方山区的重要传疟媒介。对来自云南昭通、景洪、元阳、潞西和镇康等５个地区的２５只雌按蚊，及４只雄按蚊进行随机扩增多态ＤＮＡ分析。从使用的２０个随机引物中，选择其中扩增谱带清晰的１２个引物进行分析。结果发现，只有２个引物获得的ＲＡＰＤ谱带呈单型，其余均表现为不同程度的多态性。ＵＰＧＭＡ法构建的分子系统树表明该２９只微小按蚊实际上可以归并为显著不同的５个组，分别对应于它们的地理来源，说明云南微小按蚊群体间的基因流程度不高，不同地理群体间存在显著的遗传分化。推测这种分化可能与云南复杂的生态地理环境及微小按蚊迁移能力较低等因素有关。
お? Anopheles minimus is one of main vectors of malaria in southeast Asia Recent studies have been focused on morphology,ecology,and contagion of plasmodiums In this paper,random amplified polymorphic DNA markers(RAPDs)were used to estimate genetic relationships of 29 Anopheles minimus from 5 geographic populations in Yunnan,China Among 29 individuals,four were males For twelve arbitrary primers analyzed,two of them gengrates monophic loci Out of a total of 57 RAPD bands scored,37 were varied Molecular phylogenetic tree derived from genetic distance suggested that the RAPDs were more related to geographic condition than to sexual distinction,There were genetic divergences among different geographic populations Furthermore,it was implied that RAPD was one of prospering genetic markers for studying taxonomy and evolution of Anopheles minimus
基金
国家杰出青年科学基金
云南省科委"二百人计划"
世界卫生组织热带病研究与培训规划处和云南省卫生厅的部分资助
关键词
RAPD
微小按蚊
遗传分化
Anopheles minimus,RAPD,Genetic divergence
