摘要
用筛选的高产ＳＯＤ酵母Ｙ－２１６进行发酵培养，分离提纯出ＳＯＤ，并对其性质进行了初步的研究．确定了发酵培养后的ＳＯＤ提取过程：甲苯破壁法裂解酵母Ｙ－２１６得ＳＯＤ粗提液，并通过ＤＥＡＥ－纤维素－３２柱层析及超滤等方法，得纯化的超氧化物歧化酶（ＳＯＤ），此酶部分等电点为４５，最大紫外光吸收波长为２６０ｎｍ，耐热性较好，Ｍｎ２＋可能是其金属辅基．
This was a Perliminary research for the property of the superoxide dismutases (SOD).SOD were extracted form the Yeast-216 with a good yleld.The procedure was as follows after the cell tissue culture,toluene was used to break cells to obtain a raw porduct which was purified with column chromatograophy of DEAE-collulose-32 and superfilrtate etc.One of the isoelectric points (pI) for the enzymes is 4.5,and its maximum absorption wavelength is at 260nm.The effect of metal ions on the SOD activity shows Mn 2+ as its presthetic group.
出处
《河南大学学报（自然科学版）》
CAS
1998年第1期37-40,共4页
Journal of Henan University:Natural Science
基金
河南省自然科学基金
