摘要
目的：建立高效液相色谱法测定小儿惊风七厘散中黄芩苷的含量。方法：采用shim—packCLC—ODS色谱柱（6mm×150mm），流动相：甲醇-水-冰醋酸（45：55：0．5），流速1．0mL／min，柱温40℃，进样量10μL，检测波长277nm。结果：黄芩苷在进样量0．01—0．07mg／mL范围内浓度与峰面积线性关系良好，回归方程为Y=1486379X-3748（r=0．9999，n=7），平均加样回收率为103．12％，RSD为1．76％。结论：本方法快速、简便、结果准确，可用于控制该制剂的质量。
Objective：To establish an high performance chromatography （HPLC） method to assay the concentration of baicalin in Xiao＇er Jingfeng Qili San. Methods： Shim-pack CLC-ODS （6 mm × 150 mm） column and a mobile phase of methanol, water and glacial acetic acid （45： 55： O. 5） was used with the flow rate of 1.0 mL/min and the column temperature of 40 ℃. The detection wavelength was 277 nm. Results ： There was a linear relationship within the range of 0.01 - 0.07 mg/mL and the regression equation was Y = 1486379X-3748 （r = 0.9999, n = 7）. The recovery rate was 103.12% and RSD was 1.76%. Conclusions： This method is simple and accurate and able to control the quality of the preparation.
出处
《儿科药学杂志》
CAS
2009年第1期41-43,共3页
Journal of Pediatric Pharmacy
关键词
高效液相色谱法
小儿惊风七厘散
黄芩苷
High performance liquid chromatography
Xiao＇er Jingfeng Qili San
Baicalin
