摘要
目的探讨脱氧野尻霉素（Deoxynojirimycin，DNJ）及其衍生物忖丁基-脱氧野尻霉素（N-butyl—De—oxynojirimycin，N—butyl—DNJ）的体外抗乙型肝炎病毒（HBV）作用。方法以HepG22．2．15细胞为模型，与药物共培养观察9d，于第3、6、9d收集培养上清行乙型肝炎表面抗原及e抗原（HBsAg及HBeAg）、HBV DNA定量检测。结果培养第9d，1000μg／mL DNJ、100μg／mL N-butyl-DNJ具有较明显抑制病毒复制的作用，与对照组比较，差异均具显著性（均P=0．001）。结论DNJ及其衍生物在实验浓度下无明显和直接的细胞毒性作用，分别在1000μg／mL、100μg／mL具有较明显的抑制HBV复制的作用。
Objective To evaluate the inhibitory activities of deoxynojirimycin （DNJ） and its derivate N-butyl-deoxynojirimycin （N-butyl DNJ） against hepatitis B virus（HBV） in vitro. Methods HepG2 2. 2. 15 cell were treated with DNJ. At 3rd, 6th and 9th day after treatment, the supernatant was collected for HBsAg, HBeAg and HBV DNA quantitative assay. Results At 9th day of incubation, 1 000 μg/mL DNJ and 100 μg/mL N-butyl-DNJ had obvious inhibitory effect on the replication of virus, there were significant differences compared with control group （both P = 0. 001 ）. Conclusion DNJ and its derivate have no obvious and direct cytotoxicity effect at the experimental concentration, 1 000 μg/mL DNJ and 100 μg/mL N-butyl DNJ can inhibit the replication of HBV in HepG2 2. 2. 15 cell.
出处
《中国感染控制杂志》
CAS
2009年第1期3-6,17,共5页
Chinese Journal of Infection Control
