摘要
目的观察手汗症患者胸交感神经干脑源性神经营养因子（BDNF）和神经调节因子-1（NRG-1）基因表达及对有髓神经纤维密度和单个纤维横截面积的影响，探讨与手汗症发病机制的关系。方法采用核固红一固绿髓鞘染色法显示有髓神经纤维，通过显微图像分析系统观察30例手汗症患者T3胸交感神经干有髓神经纤维密度和单个有髓神经纤维的横截面积，以及用逆转录-聚合酶链反应（RT-PCR）法检测BDNF和NRG-1基因表达强度，并与8例非手汗症患者进行对照研究。结果手汗症患者T3胸交感神经干中有髓神经纤维密度和单个有髓神经纤维的横截面积较非手汗症患者明显增高（t=7．023，P〈0．05；t=7．462，P〈0．05）；BDNF和NRG．1基因在手汗症患者r13胸交感神经于中mRNA水平的相对表达强度比值分别为1．17600±0．02870、1．21610±0．07539，较非手汗症患者的比值1．03750±0．05379、1．04270±0．04357明显增高（t=9．940，P〈0．05；t=6．195，P〈0．05）。结论BDNF和NRG-1基因表达强度增高促使胸交感神经干的有髓神经纤维密度增高以及单个有髓神经纤维的横截面积增大，从而导致胸交感神经传导速度增快、兴奋性增高，可能为手汗症的发病基础之一。
Objective To investigate the gene expression of brain-derived neurotrophic factor （BD- NF） and neuregulin-1 （NRG-1） in thoracic sympathetic trunk and their relation to myelinated nerve fiber density and single myelinated nerve fibers cross-sectional area in palmar hyperhidrosis. Methods Fast redfast green myelin sheath staining was used to show myelinated nerve fibers. Using the micro-image analysis system,30 cases of myelinated nerve fiber density and single myelinated nerve fibers cross-sectional area were observed in 373 thoracic sympathetic trunk of patients with palmar hyperhidrosis. BDNF and NRG-1 gene expression was also analyzed by RT-PCR. Eight cases of non-palmar hyperhidrosis patients served as controls. Results In T3 thoracic sympathetic trunk of patients with palmar hyperhidrosis, myelinated nerve fiber density and single myelinated nerve fibers cross-sectional area were significantly increased as compared with controls （t --7.023, P 〈 O. 05 ;t = 7.462, P 〈 0.05 respectively）. The expression of B DNF and NRG-1 in T3 thoracic sympathetic trunk of patients with palmar hyperhidrosis was 1. 176 00±0.028 70,1. 216 10± 0. 075 39 respectively ,and that in control group was 1. 037 50±0.053 79,1. 042 70±0. 043 57 respectively. The former was significantly higher than the latter （t = 9. 940,P 〈 O. 05;t=6. 195, P 〈 O. 05 respectively）. Conclusion BDNF and NRG-I gene over expression increased myelinated nerve fiber density and single myelinated nerve fibers cross-sectional area of thoracic sympathetic trunk in patients with palmar hyperhidrosis. Thus transmission speed and ability of excitatory of thoracic sympathetic nerve were also increased, which may play a role in the pathogenesis of palmar hyperhidrosis.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2009年第2期226-228,F0003,共4页
Chinese Journal of Experimental Surgery
基金
基金项目：福建省科技厅重点资助项目（2007Y0015）
福建省教育厅B类项目（JB07121）
福建省自然科学基金资助项目（2006J0088）
