摘要
在室内采用静置培养法研究了光照强度和盐度对红色和绿色品系长心卡帕藻Kappaphycus alvarezii生长的影响,主要测定了不同光照强度(30、65、110、150和200μmol.(m2.s)-1)和不同盐度(23、28、32、35和41)对这2种藻的生长率、成活率、色素含量及其吸收光谱的影响。红色品系长心卡帕藻适宜的光照强度范围为30—65μmol.(m2.s)-1,光照强度为30μmol.(m2.s)-1时培养,其成活率和日生长率都最高,分别达100%和1.25%,强光下培养生长率和成活率明显下降;红色品系长心卡帕藻适宜的盐度范围为28—32,最适为32。绿色品系长心卡帕藻适宜150—200μmol.(m2.s)-1的强光,150μmol.(m2.s)-1光照强度下培养日生长率最高,达1.05%,各种光照强度下培养其成活率变化不大;绿色品系长心卡帕藻适宜的盐度范围为23—32,最适为23。不同光照强度和盐度条件下培养,长心卡帕藻体内的色素含量会发生变化,而色素含量与其生长率有或没有明显的相关关系。不同光照强度、盐度条件下培养,藻体内的色素吸收光谱特性不变。以上研究结果对我国长心卡帕藻的栽培生产具有一定的指导意义。
Effects of different light intensity （30, 65, 110, 150 and 200umol·（m^2·s）^-1） and salinity （23, 28, 32, 35 and 41 psu） on the growth of red and green forms of Kappaphycusalvarezii were studied, and their growth rates, surviving rates, pigment contents and their absorbance spectra were determined by stationary culture method in laboratory. The results show that the red form of K. alvarezii grows better at light intensities between 30 umol·（m^2·s）^-1） and 65 umol·（m^2·s）^-1） , while the green form grows better between 150 umol·（m^2·s）^-1） and 200 umol·（m^2·s）^-1）. The optimum light intensity for the red form of K. alvarezii is 30 umol·（m^2·s）^-1） , under which the surviving rate reaches 100% and the daily growth rate reaches 1.25% when it is cultured for 28 days. When the red form is cultured at higher light intensity, its growth rate starts to decline. The optimum light intensity for the green form is 150 umol·（m^2·s）^-1） , and its highest daily growth rate can reach 1.05%; its surviving rate changes little at different light intensity. The suitable salinities are 28-32 psu and 23 32 psu, and the optimum salinities are 32 psu and 23 psu for the growth of red and green forms, respectively. The pigment contents of K. alvarezii change with light intensity and salinity, and there is some or no obvious correlation between the pigment content and the growth rate. The pigment contents of the red form are commonly higher than those of the green form. There is no change in the pigment spectrum characteristics when they are cultured at different light intensity and salinity. The extracted chlorophyll a has two absorbance peaks at 432 nm and 663 nm, respectively, and the extracted phycoerythrin has three absorbance peaks at 498 nm, 564 nm and 533 nm, respectively.
出处
《热带海洋学报》
CAS
CSCD
北大核心
2009年第1期74-79,共6页
Journal of Tropical Oceanography
基金
上海浦江人才计划项目(05PJ14086)
上海市教委优势(重点)学科资助项目(Y1101)
