摘要
目的观察肺出血新生大鼠肺组织6-酮-前列腺素(6-k-PG)、血栓素B2(TXB2)的动态变化,探讨新生儿肺出血的发病机制。方法新生大鼠40只,随机分为实验1组、实验2组、实验3组及正常对照组,每组10只。实验1、2、3组大鼠背部皮下注射1g/L肾上腺素注射液0.5mL/kg后放4℃冷室。实验1组1h后拿出,断头处死;实验2组4h拿出,断头处死;实验3组4h拿出后复温,同时注射肾上腺素1次,剂量不变,间隔1h再注射1次,剂量加倍,出冷室后2h断头处死。肉眼观察其肺组织大体病理情况,将结果分为5级:Ⅰ级正常肺,Ⅱ级肺水肿,Ⅲ级点状肺出血,Ⅳ级局灶性肺出血,Ⅴ级弥散性肺出血。采用放射免疫法测定其肺组织6-k-PG和TXB2水平。结果1.肺大体病理改变:实验1组Ⅱ级肺9只、Ⅴ级肺1只;实验2组Ⅱ级肺6只、Ⅴ级肺4只;实验3组Ⅲ级肺4只、Ⅳ级肺1只、Ⅴ级肺5只;正常对照组大鼠Ⅰ级肺10只,组间比较差异有极显著意义(P<0.01)。Ⅴ级肺电镜下见肺泡腔内有出血,部分气血屏障结构模糊。2.实验1组肺组织TXB2水平与实验2组、实验3组及正常对照组比较均明显下降(Pa<0.05)。实验1组6-k-PG/TXB2值与实验2组、实验3组及正常对照组比较均明显增高(Pa<0.05)。结论在新生大鼠肺出血的形成过程中,前列环素(PGI2)作用相应增加,PGI2使血管通透性增加,参与肺出血的发生。
Objective To observe the changes of thromboxane B2（TXB2）and 6 -keto - prostaglandin （6 -k -PG ）in the lungs of the newborn rats with pulmonary hemorrhage and explore the pathogenesis of pulmonary hemorrhage in newborn infants. Methods Forty newborn rats were divided randomly into 4 groups ：3 experimental groups（ experiment 1 group, experiment 2 group, experiment 3 group） and a normal control group （n = 10 each group）. The rats in experiment 1 group, experiment 2 group, experiment 3 group were given subcutaneous injection of 1 g/L epinephrine at a dose of 0.5 mL/kg and were put into a refrigerator of 4 ℃. The rats in experiment 1 group were taken out of the refrigerator after 1 h and were killed. The rats in experiment 2 group and experiment 3 group were taken out after 4 h. The rats in experiment 2 group were killed. The rats in experiment 3 group were rewarmed and were given epinephrine at an equal dose and were given epinephrine again after 1 h at a dose of 1.0 mL/kg, after 1 h, they were killed. Gross anatomical findings of the lungs were divided into 5 grades ： grade Ⅰ（ norreal lung）, grade Ⅱ （ pulmonary edema）, grade Ⅲ （ spotty pulmonary hemorrhage ）, grade Ⅳ （ local pulmonary hemorrhage ） and grade Ⅴ （ diffuse pulmonary hemorrhage）. TXB2 and 6 - k - PG in the lungs were nleasured by radioimmunoassay. Results 1. The pathologic changes of the lungs were revealed in 9 cases of grade Ⅱ and 1 case of grade Ⅴ in experiment 1 group;6 cases of grade Ⅱ and 4 cases of grade Ⅴ in experiment 2 group;4 cases of grade Ⅲ,1 case of grade Ⅳ and 5 cases of grade V in expriment 3 group;10 cases of grade Ⅰ in coutrol group. By the RIDIT analysis significant difference was found（P 〈 0.01 ）. Electron microscopy exam showed erythrocytes in the alveoli and blurred structure of part blood - gas barrier for grad V. 2. The level of TXB2 was significantly lower in experiment 1 group than that in experimnet 2 group ,experimnet 3 group and control group（Pa 〈0.05）. The rate of 6 - k - PG/TXB2 was higher significantly in experimnet 1 group than those in experimnet 2 group, experimnet 3 group and control group （ Pa 〈 0.05 ）. Conclusions During the formation of pulmonary hemorrhage the effect of prostacyclin increased transiently. Prostacyclin can increase permeability of vessels and take part in formation of pulmonary hemorrhage.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2009年第2期124-125,139,共3页
Journal of Applied Clinical Pediatrics
基金
武汉市卫生局临床重点学科研究项目资助(2004-241)
关键词
血栓素B2
前列环素
肺疾病
出血
大鼠
新生
thromboxane B2
prostacyclin
lung disease
hemorrhage
rat, newborn
