摘要
目的研究ｃｍｙｃ反义寡核苷酸对ＨＬ６０细胞中ｃｍｙｃ基因的表达及ＨＬ６０细胞分化的影响。方法采用针对原癌基因ｃｍｙｃ的ｍＲＮＡ５’非翻译区的一个１８ｂｐ的反义寡核苷酸５’ｄ（ＣＴＴＣＴＣＧＡＧＧＣＡＧＧＡＧＧＧ）３’与人早幼粒白血病细胞系ＨＬ６０细胞共培养５ｄ，检测ｃｍｙｃｍＲＮＡ量的变化及其对ＨＬ６０细胞分化的影响。结果用ＮｏｒｔｈｅｒｎＢｌｏｔ检测出ＨＬ６０细胞ｃｍｙｃｍＲＮＡ的含量减少；细胞形态学变化以及非特异性酯酶（ＮＳＥ）染色、硝基四氮唑蓝（ＮＢＴ）还原试验均提示ＨＬ６０细胞出现了分化，部分细胞向粒细胞方向分化，部分细胞向单核细胞方向分化。结论ｃｍｙｃ反义寡核苷酸能够抑制ｃｍｙｃ基因的表达并能诱导ＨＬ６０细胞分化。
PURPOSE To study the effect of an antisense oligodeoxynucleotide on the c myc gene expression and HL60 cells differentiation. METHODS Photomicrograph was used to show the morphology of the Wright's stained HL60 cells, Northern Blot to Measure the level of RNA, and NSE and NBT to detect the differentiation. RESULTS The expression of c myc gene mRNA was inhibited by the antisense oligodeoxynucleotide. Cell morpological changes and differentiation suggest that HL60 cells differentiate along two pathways: some into monocytes and some into granulocytes. CONCLUSIONS C myc antisense oligonucleotide can inhibit the expression of c myc mRNA and induce the differentiation of HL60 cells.
出处
《上海医科大学学报》
CSCD
1998年第3期167-170,共4页
Journal of Fudan University(Medical Science)
