摘要
研究了不同光照强度对雨生红球藻(Haematococcus pluvialis)叶绿素荧光特性及虾青素含量的影响。测定的主要参数有PSⅡ的最大光能转化效率(Fv/Fm)、PSⅡ的潜在活性(Fv/Fo)、PSⅡ的实际光能转化效率(ΦPSⅡ)、光合电子传递效率(ETR)、光化学淬灭(qP)、非光化学淬灭(NPQ)、细胞密度、叶绿素相对含量以及虾青素含量。单因子方差分析结果表明,在整个培养周期中,光照强度对雨生红球藻各叶绿素荧光参数、叶绿素相对含量、细胞密度以及虾青素含量均有显著影响(P<0.05)。多重比较结果表明,雨生红球藻的最适生长光照强度是50μmo.lm-.2s-1,当光照强度为300μmol.m-.2s-1时单个细胞中的虾青素含量均显著高于其它处理组(P<0.01),但是单位体积虾青素含量以50μmo.lm-.2s-1处理组为最高。相关性分析结果表明,在整个培养周期内,叶绿素相对含量与细胞密度均成显著正相关关系。荧光参数(Fv/Fm、Fv/Fo、ΦPSⅡ、ETR、qP、NPQ)、叶绿素相对含量以及细胞密度与光照强度的相关性则随着培养天数的不同而变化。
In this paper, the effects of different illumination on the chlorophyll fluorescence parameters and astaxanthin content of Haematococcus pluvialis were examined. The main parameters determined in the present study including the maximal photochemical efficiency of PSⅡ （ Fv/Fm）, the potential activity of PSⅡ（ Fv/Fo ）, the actual photochemical efficiency of PSⅡ in the light （ ФPS Ⅱ ）, the electron transport rate （ETR） , the photochemical quenching （qP）, the non-photochemical quenching （NPQ）, cell density, relative chlorophyll content and astaxanthin content. One-way analysis of variance showed that illumination had significant effects on the fluorescence parameters, relative chlorophyll content, cell density and astaxanthin content of H. pluvialis during the entire experimental period （ P 〈 0. 05 ）. Multiple comparisons show that the optimal growth illumination of H. pluvialis under the experimental conditions was 50 μmol.m^-2.s^-1. The astaxanthin content （ μg.cell^-1 ） at 300 μmol-m^-2.s^-1 were significantly higher than that in other treat- ments. But the astaxanthin content （ μg.mL^-1 ） at 50 μmol.m^-2.s^-1 was highest in all treatments. The analysis of correlations showed that there were significant positive correlations between relative chlorophyll content and cell density during the entire experimental period. Moreover, some fluorescence parameters （Fv/Fm, Fv/Fo, ФPSⅡ , ETR, qP, NPQ）, relative chlorophyll content and cell density all showed significant correlations with illumination, the pattern and strength of the correlations varied with culture age days.
出处
《南方水产》
2009年第1期1-8,共8页
South China Fisheries Science
基金
"十一五"国家科技支撑计划项目(2006BAD09A03)
关键词
雨生红球藻
光照强度
叶绿素荧光参数
虾青素
Haematococcus pluvialis
illumination
chlorophyll fluorescence parameters
astaxanthin content
