摘要
目的探讨血管紧张素Ⅱ-1型受体（AT1R）基因A^1166-C、血管紧张素转换酶（ACE）基因插入／缺失（I／D）和醛固酮合成酶（CYP11B2）基因-344T／C位点多态性与妊娠期高血压疾病（HDCP）的相关关系。方法采用聚合酶链反应-限制性内切酶片段长度多态性技术（PCR-RFLP），分别检测HDCP组86例和正常对照组175例AT1R基因A1166-C、ACE基因I／D和CYP11B2基因-344T／C突变位点的基因型。结果HDCP组和正常对照组AT1R基因A1166-C、ACE基因L／D和CYP11B2基因-344T/C多态性18种组合的分布不同，构成比不同；这18种组合中，相对于AT1R-AA＋ACE-Ⅱ＋CYP11B2-TT基因型，携带AT1R-AA＋ACE-DD＋CYP11B2-TC基因型人群的OR值为7．289；携带AT1R-AC＋ACE-ID＋CYP11B2-TC基因型人群的OR值为5．315；携带AT1R-AC＋ACE-DD＋CYP11B2-TC基因型人群的OR值为5．694。其余联合基因型，差异均无统计学意义（P均〉0．05）；或者由于样本量小，不具有代表性。结论HDCP组和正常对照组AT1R基因A1166-C、ACE基因I／D和CYP11B2基因-344T／C多态性18种组合中，AT1R-AA＋ACE-DD＋CYP11B2-TC联合基因型、AT1R-AC＋ACE-ID＋CYP11B2-TC联合基因型、AT1R-AC＋ACE-DD＋CYP11B2-TC联合基因型可能增加HDCP的遗传易感性；HDCP的发生，可能是多个基因共同作用的结果。
Objective To explore the relationship among genetic polymorphism of angiotension Ⅱ type 1 receptor （AT1 R） A1166-C, angiotensin converting enzyme （ ACE ） insertion/deletion （ I/D ）, aldosterone synthase （CYP11B2）-344T/C and hypertensive disorder complicating pregnancy. Methods Polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） assay was used to detect the genotypes of AT1R A1166-C, ACE （I/D）, CYP11B2 -344T/C in 86 cases of hypertensive disorder complicating pregnancy and 175 cases of normal control. Results There was 18 combined types in hypertensive disorder complicating pregnancy cases and normal control cases. Compared to AT1R-AA ＋ ACE-Ⅱ ＋ CYP11B2-TF, Odds ratios （OR） of ATl R-AA ＋ ACE-DD ＋ CYP11B2-TC, AT1 R-AC ＋ ACE-ID ＋ CYP11B2-TC and AT1R-AC ＋ ACE-DD ＋ CYP11B2-TC are 7. 289,5.315 and 5. 694 respectively. There was no statistical significance among the others. Conclusion In all 18 kinds of combined types, ATIR-AA ＋ ACE-DD ＋ CYP11B2-TC, AT1 R-AC ＋ ACE-ID ＋ CYP11B2-TC and AT1R-AC ＋ ACE-DD ＋ CYP11B2-TC might increase the susceptibility of hypertensive disorder complicating pregnancy. It is possible that multigenes are interacted in the etiology of hypertensive disorder complicating pregnancy.
出处
《中国综合临床》
2009年第2期121-123,共3页
Clinical Medicine of China
基金
河北省科学技术研究与发展指导计划（0527611008）
