摘要
目的研究建立连钱草高效液相色谱(HPLC)指纹图谱分析方法,为其质量控制和药材鉴别提供依据。方法利用高效液相色谱法,采用反相Symmetry C18柱(4.6 mm×250 mm,5μm);流动相为乙腈和0.4%磷酸溶液(梯度洗脱);检测波长327 nm;柱温30℃;流速1.0 ml/min。进样体积20μl。结果在选定的色谱条件下确定18个峰构成连钱草药材的指纹特征,各批次药材均具有上述特征,但特征峰的相对含量分布差异导致色谱概貌存在一定差异。确定了连钱草药材HPLC中绿原酸、木犀草素二个黄酮成分所对应的保留时间一致;10个批次的连钱草药材HPLC指纹图谱相似度均大于0.93。结论该方法准确、简单、稳定,其色谱指纹图谱可用于连钱草的鉴别和质量控制。
Objective To establish an HPLC fingerprint of Herba Glechonme. Methods The HPLC method was used, and the chromatography conditions were Symmetry C18 column（ 250 mm×4.6 mm,5μm） with mobile phase of acetonitrile- -0.4% phosphoric acid dihydrogen phosphate solution of water ; the UV detection wavelength was 327 nm and column temperature was 30℃ with the flow rate of 1.0 ml/min;the injection volume was 20μl. Results In this chromatography condition, eighteen peaks were identified as the characteristic fingerprints of Herba Glechomae. All samples tested contained the same eighteen peaks, but the content of each peak showed great difference among the samples ,which resuhed in the differences in their chromatograms. The retention time of Caffeotannic acid and luteolin in Herba Glechomae were determined. The fingerprint showed high similarity in samples from different habitats. Conclusion The method is exact,simple and accurate, and can be used for the identification and quality control of Herba Glechomae.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2009年第1期113-115,共3页
Lishizhen Medicine and Materia Medica Research
基金
江苏省公益研究项目(No.BM2004525)
