摘要
【目的】揭示CO2和O3浓度升高及其复合作用对植物活性氧(ROS)代谢及抗氧化酶活性的影响机理。【方法】以春小麦(Triticum aestivum L.)为试材,利用开顶式气室(OTCs)研究CO2和O3浓度升高及其复合作用下,春小麦叶片膜脂过氧化程度,活性氧产生速率、含量及抗氧化酶活性的变化。【结果】在整个生育期内,与对照相比,高浓度CO2[(550±20)μmol·mol-1]处理下,春小麦叶片相对电导率、MDA含量减小,产生速率、H2O2含量下降,SOD、CAT、POD和APX活性增强;而在O3浓度为(80±10)nmol·mol-1的条件下,春小麦叶片相对电导率、MDA含量增大,产生速率、H2O2含量升高,SOD、CAT、POD和APX活性总体上有所减弱;CO2和O3浓度升高复合[(550±20)μmol·mol-1+(80±10)nmol·mol-1]处理下,春小麦叶片MDA含量、产生速率和SOD活性总体上低于对照,而相对电导率、H2O2含量以及CAT、POD和APX活性总体上增加。【结论】CO2浓度升高抑制了春小麦叶片活性氧的代谢速率,提高了抗氧化酶的活性,对春小麦表现为保护效应,而O3浓度升高促进了春小麦叶片活性氧的代谢速率,降低了抗氧化酶的活性,对春小麦表现为伤害效应。在CO2和O3浓度升高复合处理下,CO2浓度升高在一定程度上缓解了O3浓度升高对春小麦的伤害效应,而O3浓度升高亦在一定程度上削弱了CO2浓度升高对春小麦的保护效应。
[ Objective ] The objective of this experiment is to reveal the effect mechanism of elevated carbon dioxide （CO2） and ozone （O3） concentration singly or in combination on plants reactive oxygen species （ROS） metabolism and anti-oxidative enzymes activities. [ Method ] Open-top chambers （OTCs） were utilized to investigate the change of lipid peroxidation extent, ROS production rate, content and anti-oxidative enzymes activities in spring wheat （Triticum aestivum L.） leaves under elevated CO2 and 03 concentration singly or in combination treatments. [Result] The results indicated that the elevated CO2 concentration at （550±20） μmol·mol^-1 induced a decrease on relative electrical conductivity, content of malondialdehyde （MDA）, superoxide anion （O2^-） production rate, hydrogen peroxide （H2O2） content and a increase on the activities of superoxide dismutase （SOD）, catalase （CAT）, peroxidase （POD）, ascorbate peroxidase （APX） in spring wheat leaves during the whole growth stage. While the elevated O3 concentration at （80±10） nmol·mol^-1 showed the opposite effects, which resulted in higher relative electrical conductivity, MDA content, O2^- production rate, H2O2content and lower activities of SOD, CAT, POD, APX in spring wheat leaves compared with the control. But under the combined treatment of CO2 and O3 at concentration of （550±20） μmol·mol^-1＋（80±10） nmol·mol^-1, MDA content, O2^- production rate, SOD activity were higher in general compared with the control. While relative electrical conductivity, H2O2 content and the activities of CAT, POD, APX increased in the leaves of spring wheat. [Conclusion] ROS metabolism rates are restrained and anti-oxidative enzymes are enhanced by elevated CO2 concentration, which presents protective efficacy on spring wheat. While elevated O3 concentration accelerates ROS metabolism rates and reduces anti-oxidative enzymes activities, which represents injured effects on spring wheat. In combination of elevated CO2 and O3 concentration, the beneficial effects of CO2 on spring wheat are more than compensating the negative effects imposed by O3 and the latter in its turn partly counteracted the positive effects on spring wheat of the former.
出处
《中国农业科学》
CAS
CSCD
北大核心
2009年第1期64-71,共8页
Scientia Agricultura Sinica
基金
国家自然科学基金资助项目(30500069,30570348)
关键词
CO2浓度升高
O3浓度升高
复合作用
春小麦
活性氧
抗氧化酶
elevated CO2 concentration
elevated O3 concentration
combination
spring wheat
ROS
anti-oxidative enzymes
