摘要
目的:探讨重组慢病毒介导的基质金属蛋白酶-9(MMP-9)基因沉默对喉鳞状细胞癌移植瘤生长的抑制作用。方法:构建喉癌移植瘤模型,采用RNA干扰技术,瘤内注射MMP-9-RNAi-Lentivirus重组慢病毒,以空病毒载体作为对照,观察抑瘤效果。运用Western-blot方法检测移植瘤中MMP-9蛋白表达。运用免疫组织化学方法检测移植瘤中增殖细胞核抗原(PCNA)的表达,评估其对细胞增殖的影响。结果:重组慢病毒MMP-9-RNAi-Lentivirus治疗后,治疗组裸鼠移植瘤平均重量为(1.484±0.391)g,明显小于对照组(2.618±0.465)g(P<0.05)。治疗组肿瘤平均体积为(1.177±0.270)cm3,明显小于对照组(2.034±0.366)cm3(P<0.05),抑瘤率为43.32%。Western-blot结果显示治疗组MMP-9蛋白表达阴性7例,弱阳性3例,对照组MMP-9蛋白表达均阳性。免疫组织化学显示治疗组PCNA指数为(55.41±8.77)%,显著低于对照组(77.04±6.91)%(P<0.05)。结论:MMP-9-RNAi-Lentivirus重组慢病毒能有效抑制喉癌移植瘤生长及增殖。
Objective： To investigate the inhibitive role of lentivirus mediated MMP-9 gene silence in the growth of laryngeal cancer xenografts. Method： The nude mouse model of laryngeal squamous carcinoma was estab lished and RNA interference （RNAi） technic was used. Recombinant MMP-9 RNAi-Lentivirus was intratumoral injected while the empty vector lentivirus was taken as control. After the treatment, tumor inhibitive effect was observed and western blot was used to test the expression of MMP-9 in the xenografts. Finally, the PCNA expression in xenografts were examined with immunohistochemistry to evaluate proliferation change of the Hep-2 cell, s. Result： The average tumor weight was （1. 484±0. 391）g in group treated with MMP-9 RNAi-Lentivirus which was significantly lower than that （2. 618±0. 465）g in the control group （P≤0. 05）. The average tumor vol- ume was （1. 177±0. 270）cm3 in group treated with MMP-9 RNAi-Lentivirus which was significantly lower than that （2. 034±0. 366）cma in the control group（P〈0.05） and the tumor inhibitive rate was 43, 32%. Western-blot showed there were 7 cases of xenografts with MMP-9 protein negative expression and the other 3 cases were tested with weak positive expression in the treated group xenografts. Whereas all the 10 cases of xenografts were tested with MMP 9 positive expression in the control group. Immunohistochemist showed PCNA index in treated group was （55.41±8.77）% which was obviously lower than that （77.04±6.91）% in control group（P〈0.01）. Conclusion：The growth and proliferation of laryngeal cancer could be inhibited by recombinant MMP 9-RNAi-Lentivirus.
出处
《临床耳鼻咽喉头颈外科杂志》
CAS
CSCD
北大核心
2008年第24期1129-1131,共3页
Journal of Clinical Otorhinolaryngology Head And Neck Surgery
基金
国家自然科学基金资助(No:30540010)
中国博士后科学基金(No:20070410920)
黑龙江省青年基金(No:QC06C054)
黑龙江省教育厅海外学人科研基金(No:1151hz029)
哈尔滨市科技创新人才基金(No:2006RFLXS028)
哈医大二院博士启动基金(No:BS2006015)
