摘要
目的：探讨抑癌基因ｐ５３与壶腹周围癌组织学分级和预后的关系。方法：用单克隆抗ｐ５３蛋白抗体（Ｄｏ－７）通过免疫组化ＬＳＡＢ法对６１例壶腹周围癌进行检测。结果：ｐ５３蛋白在壶腹周围癌中有较高的表达，阳性率为６７．２％（４１／６７）；组织学分级呈高分化组阳性率５１．７％（１５／２９），明显低于中低分化组阳性率８１．３％（２６／３２）（Ｐ＜０．０１）；术后３年生存率为４７．５％（２９／６１），其中术后３年内死亡组阳性率８４．４％（２７／３２），明显高于存活满３年组阳性率４８．３％（１４／２９）（Ｐ＜０．０１）。结论：ｐ５３蛋白的表达与壶腹周围癌的组织学分级成正相关而与术后生存期成负相关。
Objective: To determine the relationship between the expression of p53 mutant protein and its prognostic significance in periampulary carcinoma. Methods: Using LSAB method with monoclonal p53 antibody Do 7, the experssion of p53 was investigated immunohistochemically in 61 periampulary carcinoma. Results: p53 expression was detectable in 67.2% (41/61) cases of periampulary carcinoma. The positive rate of p53 expression in the well differentiated group (51.7%,15/29) was lower than that in the undifferentiated group (81.3%,26/32), the difference was significant (P<0.01). The three year survival rate was 47.5%(29/61), the p53 expression rate (48.3%,14/29) in the group of more than three year survival was much higher than that (84.4%,27/32) in the group survival less than three year, the difference was significant (P<0.01). Conclusions: p53 expression in periampulary carcinoma was positively correlated with histologic grades and inversely correlated with postoperative survival duration. It may be as a useful referential indicator for valuating periampulary carcinoma in histologic grade and prognosis.
