摘要
目的:探讨进展期结直肠癌组织中IL-6 mRNA表达及其对进展期结直肠癌患者低白蛋白血症形成的影响。方法:63例患者按血清白蛋白水平<35 g/L或≥35 g/L分成两组,低白蛋白血症组(n=38),白蛋白正常组(n=25),分别使用ELISA法检测患者血清中IL-6水平和用RT-PCR方法检测癌组织中IL-6 mRNA表达水平。结果:进展期结直肠癌低白蛋白血症组血清IL-6水平和癌组织中IL-6 mRNA表达水平明显高于白蛋白正常组(P<0.05),且血清IL-6水平与白蛋白水平呈负相关(r=-0.57,P=0.00),与癌组织中IL-6 mRNA表达水平呈正相关(r=0.28,P=0.03)。结论:进展期结直肠癌合并低白蛋白血症患者的癌组织中IL-6 mRNA呈高表达。癌组织IL-6 mRNA高表达是导致血清IL-6水平增高的重要因素。
Objective： This study was conducted to clarify the levels of the IL-6 mRNA expression in cancer tissue and to further elucidate the relationship between the levels of IL-6 mRNA expression in cancer tissue and hypoalbumineamia in patients with advanced colorectal cancer. Methods： According to the serum albumin concentration, 63 patients with advanced eolorectal cancer were divided into 2 groups： hypoalbumineamia group with concentration of serum albumin〈35 g/L （38 patients） and normal group with concentration of serum albumin 935 g/L（25 pa- tients）. Serum IL-6 concentration was quantified by ELISA. The expression IL-6 in colorectal cancer tissue was quantified by RT PCR. Result： Compared with normal group, hypoalbumineamia group showed higher levels of IL-6 mRNA expression and serum IL-6 concentration in colorectal cancer tissue（P〈0.05）. Serum IL-6 concentration was negatively correlated with serum albumin concentration（r=-0.57, P=0.00）, pos itively correlated with expression levels of IL-6 mRNA in colorectal cancer tissue（ r =0.28, P =0.03）. Conclusion： There is high expression of IL-6 mRNA in advanced colorectal cancer tissue. The high level of IL-6 mRNA in colorectal cancer tissue is an important factor causing increase of serum IL-6 concentration.
出处
《广西医科大学学报》
CAS
北大核心
2008年第6期831-834,共4页
Journal of Guangxi Medical University
基金
广西自然科学基金资助项目(No.桂科2006-89)
广西卫生厅重点项目(No.桂卫重200523)
关键词
结直肠癌
低白蛋白血症
IL-6
RT-PCR
co｜orectal neoplasms
hypoalbumineamia
interleukin-6
reverse transcriptase chain reaction
