益智健脑颗粒对快速老化小鼠SAMP8海马Pin1和HMGB1 mRNA表达的影响被引量：7

Effect of yizhi jiannao granules on the expression of Pin1 and HMGB1 mRNA in the hippocampus of SAMP8 mice

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摘要目的:研究益智健脑颗粒对快速老化小鼠SAMP8海马肽基脯氨酰顺反异构酶(peptidylprolyl-cis-trans isomerase A,Pin1)和高迁移率族蛋白B1(high mobility group box1,HMGB1)mRNA表达的影响。方法:6月龄快速老化小鼠SAMP8随机分为中药治疗组和模型组,6月龄正常老化小鼠SAMR1为正常对照组。中药组以益智健脑颗粒浓缩液灌胃;正常对照组和模型组以双蒸水灌胃。8周后,采用逆转录-聚合酶链反应(RT-PCR)方法检测海马组织中Pin1和HMGB1 mRNA的表达。结果:与模型组比较,中药治疗组Pin1 mRNA相对表达量上调(P<0.05);HMGB1mRNA相对表达量下调(P<0.05)。结论:益智健脑颗粒可能通过上调Pin1 mRNA表达,下调HMGB1 mRNA表达,来达到防治阿尔茨海默病的作用。 Objective To investigate the effect of yizhi jiannao granule concentration fluid （ YCF ） on the expression of peptidyl-prolyl-cis-trans isomerase A （ Pin 1 ） and high mobility group box 1 （HMGB1） mRNA in the hippocampus of senescence accelerated mice Senile-Prone8 （ SAMP8 ）. Methods Six-month old SAMP8 mice were randomly divided into a YCF group and a model group. Six-month old SAMP8 mice were served as a normal control group. The YCF group was ravaged, while the model group and the normal control group were gavaged with double-distilled water for 8 weeks. The hippocampus was taken out for examination. The expression of Pin1 and HMGB1 mRNA was measured by RT-PCR. Results In the YCF group, the level of Pin1 mRNA increased, and the level of HMGB1 mRNA decreased compared with that of the model group. Conclusion Yizhi jiannao granules can prevent Alzheimer＇ s disease by increasing the Pin 1 level and decreasing the HMGB 1 level.

作者王慧玲董克礼李广诚彭贤文朱宏

机构地区长沙市精神病医院中南大学湘雅三医院中医科

出处《中南大学学报（医学版）》 CAS CSCD 北大核心 2009年第1期63-66,共4页 Journal of Central South University ：Medical Science

关键词益智健脑颗粒/中药阿尔茨海默病肽基脯氨酰顺反异构酶高迁移率族蛋白B1 yizhi jiannao granule/Chinese medicine Alzheimer＇ s disease peptidyl-prolyl-cis-trans isomerase A high mobility group box 1

分类号 R285 [医药卫生—中药学]

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