摘要
目的探讨结核分枝杆菌抗LAM、38kD、16kD、CFP10、ESAT-65项抗体的检测对临床的应用价值。方法检测50例结核病、80例非结核病患者血清,应用蛋白芯片识别仪检测受试者血清中5项结核抗体,并对诊断试验结果进行分析评价。结果130例临床标本显示,以任一抗体检测阳性诊断为结核病阳性,则诊断结核病的灵敏度为92.0%,特异度为77.5%,正确率为83.1%,Youden指数为69.5%。结论蛋白芯片联合检测5项结核抗体具有较高的诊断价值,与"金标准"诊断的一致性较好,且取材简易、操作方便、历时较短,可以作为临床上诊断结核病特别是肺外结核病的一项重要辅诊项目。
Objective To detect 5 antibodies against tuberculoprotein, LAM, CFP10, ESAT-6 and 2 proteins at molecular weight of 38 kD and 16 kD by a protein chip and explore its clinical value in diagnosis of tuberculosis. Methods The serum antibodies from 50 tuberculosis patients and 80 non-tuberculosis patients were analyzed by this protein chip, and the diagnostic value of the test was evaluated. Results As was shown in the 130 clinical samples, the sensitivity, specificity, accuracy and Youden index of the diagnostic test were 92.0% , 77.5% , 83. 1% and 69.5% respectively. Conclusion The tuberculosis protein chip assay is of high value in diagnosis of both pulmonary and non-pulmonary tuberculosis patients, and has good coincidence with gold standard. It is time-saving and easy to sampling and practicing, and can be regarded as supplemental item for non-pulmonary tuberculosis.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2009年第3期220-222,共3页
Journal of Third Military Medical University
基金
第三军医大学临床科研基金(2008)~~
关键词
结核分枝杆菌
结核抗体
蛋白芯片
mycobucterium tuberculosis
antibodies
protein chip
