摘要
目的借助微小RNA(microRNA,miRNA)芯片研究IgA肾病(IgA nephropathy,IgAN)中miRNA的差异表达。方法收集11例IgAN患者肾穿标本作为IgAN组,3例镜下病理检查正常的肾皮质作为对照组。使用Trizol试剂提取每组总RNA,分离miRNA,采用miRNA芯片筛选出差异表达显著的miRNA,荧光实时定量PCR(Real-timePCR)技术验证芯片结果的可靠性。结果IgAN组中miRNA表达出现显著差异的有66个,其中表达显著上调35个,显著下调31个。挑选hsa-miR-637、hsa-miR-492进行Real-timePCR相对定量检测,结果显示2个miRNA的表达趋势与芯片结果相符,证明了芯片结果的可靠性。结论IgAN是一种经典的多因素、多基因病,本实验通过芯片技术表明miRNA在IgAN中出现显著差异表达。
Objective To investigate the differential expressions of microRNAs in IgA nephropathy patients by using microRNAs array. Methods Samples of 11 patients with IgA nephropathy were collected by biopsy （IgA nephropathy group） , and 3 renal cortexes of eumorphism patients as normal control group. Total RNA of every group was extracted using Trizol according to the manufacturer＇ s instruction. MicroRNAs were isolated from total RNA and significant differential expression microRNAs were screened by microRNAs array. The reliability of the results of microRNAs array was validated by Real-time PCR. Results In IgA nephropathy, there were 66 microRNAs significant differential expression of which 35 up-regulated and 31 down-regulated. hsa-miR-637 and hsa-miR-492 were selected for relative quantification by Real-time PCR. It showed that the results of both methods had no significant deviation and the results of microRNAs array was reliable. Conclusion IgA nephropathy is a classic multiple factors, polygenic disease and until now there is no mechanism which can identify it perfectly. Our data indicate that microRNAs are significant differential expression in lgA nephropathy. The lucubrated of microRNAs in IgA nephropathy may help to diagnosis, treat and prevent IgA nephropathy.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2009年第3期265-268,共4页
Journal of Third Military Medical University
基金
广西自然科学基金(0832289)~~
