摘要
A novel inhibition based biosensor of glucose oxidase(GOx) for environmental mercury detection was developed. An electropolymerized aniline membrane was prepared on a platinum electrode containing ferrocene as electron transfer mediator, on which GOx was cross-linked by glutaraldehyde. The response of the sensor was based on the current reduction in the electrochemical system by inhibition of mercury against GOx electrode. The detection limit of the inhibition-based sensor for mercury is 0.49 μg/L, and the linear response ranges are 0.49-783.21 μg/L and 783.21 μg/L-25.55 mg/L. The GOx membrane can be completely reactivated after inhibition, and remains 70% of the activity in more than one month. The sensor was used for mercury determination in compost extract with good results.
A novel inhibition based biosensor of glucose oxidase（GOx） for environmental mercury detection was developed. An electropolymerized aniline membrane was prepared on a platinum electrode containing ferrocene as electron transfer mediator, on which GOx was cross-linked by glutaraldehyde. The response of the sensor was based on the current reduction in the electrochemical system by inhibition of mercury against GOx electrode. The detection limit of the inhibition-based sensor for mercury is 0.49 μg/L, and the linear response ranges are 0.49 783.21 gg/L and 783.21 μg/L-25.55 mg/L. The GOx membrane can be completely reactivated after inhibition, and remains 70% of the activity in more than one month. The sensor was used for mercury determination in compost extract with good results.
出处
《中国有色金属学会会刊：英文版》
EI
CSCD
2009年第1期235-240,共6页
Transactions of Nonferrous Metals Society of China
基金
Project(50608029) supported by the National Natural Science Foundation of China
Project(2004AA649370) supported by the Hi-tech Research and Development Program of China
Project(2005CB724203) supported by the National Basic Research Program of China
Project(IRT0719) supported by the Program for Changjiang Scholars and Innovative Research Team in University of China
关键词
有色金属
金属学
性能参数
分析
mercury
inhibition
glucose oxidase
biosensor
compost
