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忍冬藤质量标准研究 被引量:20

Studies on the Quality Control of Caulis Lonicerae Japonicae
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摘要 目的建立RP-HPLC测定忍冬藤中绿原酸、咖啡酸、当药苷和马钱子苷含量的方法,并以此对忍冬藤进行质量控制。方法色谱柱为Aglient Extend,C18(4.6mm×250mm,5μm);流动相为甲醇-0.4%醋酸水溶液(26:74);流速1.0mL·min^-1,检测波长240nm。结果绿原酸、咖啡酸、当药苷和马钱子苷的线性范围和相关系数分别为23.5~470mg.L^-1,r=0.9999;12~240mg·L^-1,r=0.9997;10.5~210mg·L^-1,r=0.9999;13-260mg·L^-1,r=0.9998。平均回收率分别为97.7%(RSD=2.23%),96.6%(2.29%),95.0%(2.26%),103.3%(2.93%)。结论本方法简单、快速、可靠,可用于忍冬藤药材的质量控制。 OBJECTIVE To establish a RP-HPLC method for simultaneous determination of four bioactive compounds in Caulis Lonicerae Japonicae, i.e. chlorogenic acid, caffeic acid, sweroside and loganin. METHODS The separation was performed on an Aglient Extend-C18 column (4.6 mm×250 mm, 5μm), using a isocratic elution with methanol-water (containing 0.4% acetic acid) as the mobile phase. The flow rate was 1.0 mL·min^-1, the detection wavelength was set at 240 nm. RESULTS The linear ranges and regression equations of chlorogenic acid, caffeic acid, sweroside and loganin were 23.5-470 mg·L^-1 with r=0.999 9; 12-240 mg·L^-1 with r=0.999 7; 10.5-210 mg·L^-1 with r=0.999 9 and 13-260 mg·L^-1 with r=0.999 8, respectively. The average recoveries (n=6) ofthe four constituents were 97.7% (RSD=2.23%), 96.6% (2.29%), 95.0% (2.26%), 103.3% (2.93%), respectively. CONCLUSION This method is simple, rapid and reliable for the quality control of Caulis Lonicerae Japonicae.
出处 《中国药学杂志》 CAS CSCD 北大核心 2009年第1期52-54,共3页 Chinese Pharmaceutical Journal
基金 国家杰出青年科学基金资助(30325046)
关键词 忍冬藤 反相高效液相色谱法 绿原酸 质量控制 CaulisLoniceraeJaponicae RP-HPLC Chlorogenicacid Quality control.
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