摘要
背景与目的:研究杜香熊果酸提取物对小鼠遗传损伤是否具有保护作用。材料与方法:以环磷酰胺(cyclophosphamide,CP)所导致的遗传损伤小鼠作为损伤模型,采用小鼠骨髓嗜多染红细胞微核试验,检测杜香熊果酸提取物对遗传损伤的保护作用。结果:杜香熊果酸提取物1.16、2.30、4.60g/kg的骨髓嗜多染红细胞微核发生率分别为2.00‰±0.37‰、2.00‰±0.45‰、1.80‰±0.37‰,与正常组(2.00‰±0.45‰)比较差异均无统计学意义(P>0.05),与阳性对照组(24.00‰±0.71‰)其差异均具有统计学意义(P<0.01)。杜香熊果酸提取物1.16、2.30、4.60g/kg加环磷酰胺组骨髓嗜多染红细胞微核发生率分别为17.20‰±1.24‰、16.40‰±1.53‰、16.00‰±0.51‰,明显低于阳性对照组(24.00‰±0.71‰),其差异均具有统计学意义(P<0.05)。结论:杜香熊果酸提取物对遗传损伤有保护作用。
BACKGROUND AND AIM： To study the protective effects of ursolic acid extract from Ledum Pulastre L. against genetic damage. MATERIALS AND METHODS： We used mice with genetic damage caused by cyclophosphamide; （CP）, as a model of injury. Through mouse ceils of bone marrow PCE micronuclear test, the protective effects of ursolic acid extract on genetic damage was measured. RESULTS： The bone marrow cells-nuclear PCE rates of ursolic acid extract at 1.16, 2.30, 4.60 g/kg dose were 2.20‰± 0.37‰, 2.00‰ ± 0.45‰, 1.80‰ ± 0.37‰, respectively. There was no significant difference with the normal group （2.00‰± 0.45‰） （P 〉 0.05）, but showed a significant difference with positive group （24.00‰±0.71‰）（P〈0.01）. The bone marrow cells-nuclear PCE rates of ursolic acid at 1.16, 2.30, 4.60 g/kg dose with added CP were 17.20‰± 1.24‰, 16.40‰± 1.53‰, 16.00‰±0.51‰, respectively, significantly different from the positive group（24.00‰ ± 0.71‰） （P 〈 0.05） . CONCLUSION： Ursolic acid extract from Ledum Pulastre L. demonstrated protective effects against genetic damage.
出处
《癌变．畸变．突变》
CAS
CSCD
2009年第1期68-69,73,共3页
Carcinogenesis,Teratogenesis & Mutagenesis
关键词
杜香
熊果酸
遗传损伤
细胞微核
Ledum pulastre L.
ursolic acid
genetic damage
cell micronucleus
