摘要
目的了解HCV 1b基因型5’-NCR基因变异株的感染状态。方法应用限制性内切酶酶切分析检测119例1b型中5＇-NCR基因变异。结果在119例HCV 1b型中存在5种感染状态：①有Mbo I切点变异株占58．0％（69／119）；②无Mbo I切点变异株占16．0％（19／119）；③有Mbo I切点和无Mbo I切点混合感染株占10．9%（13／119）；④有BamH I切点变异株占10．1％（12／119）；⑤有Mbo I双切点变异株占5．0％（6／119）。1例干扰素治疗有效的丙型肝炎患者，HCV RNA持续阴性，未检测出5’-NCR变异（有Mbo I单一切点），而2例干扰素治疗12个月和26个月的丙型肝炎患者血清ALT反复升高，HCV RNA持续阳性样品中，1例检出5’-NCR Mbo I酶切位点变异。结论研究结果提示，在丙型肝炎患者血清中存在HCV 1b型中5’-NCR基因单一的Mbo I切点的样品，58．0％可能是HCV野生株感染的状态。
Objective To investigate HCV lb genotype 5＇-NCR gene mutation of the infection. Methods Application of restriction enzyme digestion analysis detected 119 cases of type lb in the 5＇-NCR gene mutation. Results In 119 cases of type HCV lb in the five kinds of infection ：①Mbo I restriction site of 58.0% （69/119）;②Mbo I restriction site mutation of 16.0%（19/119）;③Mbo I restriction site and Mbo I restriction site mutation of mixed infection 10. 9%（13/119）;④BamH I restriction site mutation of 10. 1 % （12/119） ;⑤Mbo I double-restriction site point mutation of 5.0 % （6/119）. One case of interferon therapy effective in patients with hepatitis C,HCV RNA sustained negative,the 5＇-NCR variation （single Mbo I site） wasn＇t detected,and two cases of interferon therapy 12 months and 26 months of hepatitis C serum ALT repeatedly increased,HCV RNA sustained positive samples, one case of detection 5＇-NCR Mbo I restriction site variability. Conclu- sion Study results suggest that in patients with hepatitis C in a single Mbo I cut-off point of samples of HCV 1b genotype 5＇-NCR gene 58.0% may be the wild strains of HCV infection status.
出处
《现代检验医学杂志》
CAS
2009年第1期118-120,共3页
Journal of Modern Laboratory Medicine
基金
国家“十五”科技攻关计划资助项目（2001BA705806,2004BA718B）.
