摘要
目的:研究表达小鼠白细胞介素21(mIL-21)的Sp2/0细胞与用Sp2/0细胞预先免疫的小鼠淋巴细胞体外共培养,是否对预致敏淋巴细胞增殖及功能有影响。方法:获取灭活Sp2/0细胞免疫的小鼠淋巴细胞,在mIL-2存在的条件下,以mIL-21转染的Sp2/0细胞为刺激细胞,用流式细胞术检测CFSE标记的淋巴细胞增殖和7-AAD标记的细胞毒活性;用ELISpot法确定分泌IFN-γ的淋巴细胞数量。结果:转染mIL-21的Sp2/0细胞对预致敏的淋巴细胞增殖有明显影响,活化的淋巴细胞对靶细胞的杀伤率(39.57%±4.72%)与对照组(23.18%±2.94%)相比有较大的提高(P<0.05),且分泌IFN-γ的细胞数量明显增加。活化增殖后的淋巴细胞回输至环磷酰胺预处理的小鼠,能延长小鼠的成瘤时间。结论:表达mIL-21的Sp2/0细胞可有效促进肿瘤抗原特异性淋巴细胞活化及增殖,并增强其对肿瘤细胞的杀伤功能。
Objective: To investigate the influence on murine tumor specific lymphocytes proliferation and cytotoxicity with gene modified Sp2/0 cells expressing mIL-21 in vitro. Methods: Tumor Ag-specific lymphocytes were obtained from pre-immunized BALB/c mice with irradiated Sp2/0 cells, in presence of mIL-2, incubated with the stimulators of inacti-vated mIL-21-modified Sp2/0 cells in vitro. The proliferation and cytotoxic activities of activated lymphocytes were ana- lyzed by flow eytometry respectively staining with CFSE and 7-AAD. The number of IFN-γ-secreting cells was evaluated by ELISpot. Results: The lymphocytes from mice immunized with inactived Sp2/0 cells were obvious proliferative response to Sp2/0 cells expressing mIL-21 in vitro. The killing rate of activated lymphocytes induced by mIL-21-modified Sp2/0 cells on target cells was 35.57%±4.72% and it is increased significantly compared with control group (P〈0.05), and the numbers of Sp2/0 cells secreting IFN-γ was also significant higher than that of the control groups. The activated lympbocytes were transferred to mice pre-treated with cyclophosphamide and the time of mice growing tumor was longer than that of control mice. Conclusion: C, ene modified Sp2/0 cells expressing mIL-21 markedly promote activating and proliferating of lymphocytes from mice immunized with inactived Sp2/0 cells, and enhance cytotoxicity of activated lymphocytes to tumor cells obviously.
出处
《生物技术通讯》
CAS
2009年第1期31-34,共4页
Letters in Biotechnology
基金
江苏省六大人才高峰资助项目(D14)