摘要
目的建立HPLC测定金青感冒颗粒中绿原酸含量的方法。方法采用COSMOSIL 5C18-MS-II色谱柱,流动相为乙腈-0.5%冰醋酸(10∶90),检测波长为327nm,流速为1.0mL·min-1。结果绿原酸在0.06159～0.51325mg范围内具有良好的线性关系,r=0.99998,平均回收率为97.6%,RSD=1.13%(n=9)。结论本法简便,准确,灵敏度高,重复性好,可用于该药品成分的含量测定。
Objective To establish a HPLC method to determine Chlorogenic acid of Jinqinganmao granules by HPLC. Methods The analysis was carried out using C18 column, the mobile phase was acetonitrile-0.5% acetic acid （10 ： 90）, the detecting wavelength was at 327nm, with the flow rate of 1.0mL · min-1. Results The linear range of Chlorogenie acid was 0. 06159-0. 51325mg （r=0. 99998）, the average recovery was 97.6%, RSD=1. 13% （n=9）. Conclusions The method is simple and accurate, it can be used in the quality control of Jinqinganmao granules.
出处
《中国药事》
CAS
2009年第1期68-69,97,共3页
Chinese Pharmaceutical Affairs
