摘要
酶联免疫吸附技术(ELISA)作为先进的免疫化学检测技术正越来越广泛地应用于沙门氏菌检测中。文中介绍了沙门氏菌的特性及其主要危害,阐述了ELISA的基本原理,并结合国内外研究成果,综述了目前ELISA法在沙门氏菌检测中几种常用的方法,主要有:直接ELISA法、竞争ELISA法、Dot-ELISA法、LPS-ELISA法和PCR-ELISA法,从而表明了在沙门氏菌检测中ELISA法具有良好的特异性和敏感性。
As an advanced immunochemistry detection technique, enzyme-linked immunosorbent assay （ELISA） is broadly applied in detection of Salmonella. The characteristics of salmonella and its main hazards are introduced in this paper, as well as the basic principle of ELISA. According to international research progress, the current ELISA methods used for detection of Sahnonella are summarized, including direct ELISA, competition ELISA, Dot-ELISA, LPS-ELISA and PCR-ELISA. All these ELISA methods showed high specificity and sensitivity in detection of Salmonella.
出处
《中国酿造》
CAS
北大核心
2009年第1期1-3,共3页
China Brewing
基金
山西省科技攻关项目(2006031066-2)
山西省高校科技研究开发项目(20051219)
