摘要
对1997-2005年从国内分离到的10株水禽源新城疫病毒(NDV)进行了生物学特性和遗传特性研究。致病指数MDT和ICPI测定结果表明10株分离株均属于强毒株。采用RT-PCR扩增了各分离株F基因主要功能区片段(535 bp)并进行了序列分析。10株分离株F蛋白裂解位点的氨基酸组成均为112RRQKRF117,具有典型的强毒特征,与致病指数测定结果一致。参照国内外已发表的部分毒株的F基因序列,构建NDV的遗传进化树,分析毒株间的遗传进化关系。遗传进化树分析表明10株NDV分离株中有8株属于基因Ⅶd型,1株属于基因Ⅶc型,1株属于基因Ⅸ型。表明基因Ⅶ型NDV是造成中国水禽近年来发生新城疫的主要原因,与同期中国鸡群发生新城疫感染的流行特点一致。
Ten representative isolates of Newcas in waterfowl in China during 1997 to 2005 were tle Disease Virus （NDV） obtained from outbreaks characterized both pathotypically and genotypically. Pathogenicity tests （MDT and ICPI） showed that all ten isolates were velogenic strains. The main functional region of the F gene comprising 535 nucleotides was amplified by RT-PCR and sequenced. The amino acid sequence of the fusion protein cleavage site in all ten isolates was ^112RRQKRF^117, which is a typical sequence of velogenic strains and is in agreement with the results of in vivo pathogenicity tests. For genotyping, a phylogenetic tree based on nucleotides 47- 435 of the F gene was constructed. Phylogenetic analysis showed that, among the 10 isolates, 8 isolates were of the genotype Ⅶd virus, 1 belonged to genotype Ⅸ virus, 1 belonged to genotypeⅦc virus. Results indicate that the strains of genotype Ⅶd NDV have been the major pathogen, responsible for most epizootic ND outbreaks in waterfowl in China since 1997.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2009年第1期145-148,共4页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
青岛市科技计划项目(07-2-3-5-jch)
关键词
新城疫病毒
水禽
分子流行病学
Newcastle disease virus
waterfowl
molecular epidemiology
