摘要
目的:测定西洋参根及茎叶皂苷提取物中12种单体人参皂苷的含量。方法:采用WatersAlliance2690液相色谱仪,配Waters996型-PDA检测器,XTerraRP18色谱柱(3.9mm×150mm,5μm),乙腈(A)-水(B)为流动相,梯度洗脱[0min(20%A)→20min(20%A)→25min(25%A)→45min(26%A)→55min(27%A)→70min(32%A)→80min(47%A)→85min(53%A)→90min(53%A)],流速为1.0mL.min-1,检测波长203nm,柱温40℃。结果:12种人参皂苷Rg1、Rg2、Rg3、Re、Rd、Rh1、Rh2、Rf、Rc、Rb1、Rb2和Rb3浓度在其各自的线性范围内,与峰面积呈良好的线性关系,r值均>0.99(n=6);回收率在91.0%～100.4%范围内。结论:本方法精密度和重现性良好,适用于西洋参根及茎叶皂苷提取物中的单体人参皂苷的分析研究,可用于控制西洋参根及茎叶皂苷提取物的质量。
Objective ： To establish an RP - HPLC method for determination of the contents of twelve active constituents ginsenoside Rg1, Rg2, Rg3, Re, Rd, Rh1 , Rh2, Rf, Rc, Rb1 , Rb2 and Rba,in extract of root, and stem and leaf from Panax quinquefolius. Method：The separating was performed on a XTerra RP18 column （3.9 mm × 150 mm,5 μm）. The mobile phase was acetonitrile （A） - water （ B ）, with gradient elution [ 0 min （ 20% A） →20 min （ 20% A）→25 min（25% A）→45 min（26% A）→55 min（27% A）→70 min（32% A）→80 min（47% A）→85 min （53% A）→90 min（53% A） ] at a flow -rate of 1.0 mL · min^-1. Detection was at 203 nm,and the column temperature was set up at 40℃. Results： Rg1 , Rg2, Rg3, Re, Rd, Rh1 , Rh2, Rf, Rc, Rb1 , Rb2 and Rbahad good linear relation in their linear ranges （ r ≥ 0. 99 ） , the average recoveries were between 91.0% - 100. 4%. Conclusion： This method is accurate and reproducible as well as specific and can assess the quality of extract of root, and stem and leaf from Panax quinquefolius.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2009年第1期79-81,共3页
Chinese Journal of Pharmaceutical Analysis
关键词
西洋参
单体皂苷
RP—HPLC
extract of Panax quinquefolius L.
ginsenoside
RP - HPLC
