摘要
目的评价氟比洛芬酯预先给药对大鼠内毒素性急性肺损伤的影响。方法雄性SD大鼠40只，体重190～220g，随机分为3组：对照组（c组，n=8）、脂多糖组（LPS组，n=16）和氟比洛芬酯组（FA组，n=16）。C组尾静脉注射生理盐水（NS）1ml；LPS组尾静脉注射LPS5mg／kg（1m1）；FA组尾静脉注射氟比洛芬酯6mg／kg（1ml）后0．5h注射LPS 5mg／kg（1ml）。LPS组和FA组于注射LPS后2、4h时、C组于注射Ns后4h时，各随机处死8只大鼠，取股动脉血样8ml，行血气分析；采用放免法测定血清血栓素B2（TXB2）和6-酮-前列腺素F1α（6-keto PGF1α）的浓度；采用ELISA方法测定血清肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）、IL-6和IL-10的浓度。取左肺上叶组织，计算肺组织湿／干重比值（W／D）和肺含水量（LC）。注射LPS或Ns后4h时取左肺下叶组织，光镜下观察肺组织病理学结果。结果与C组比较，LPS组PaO2和PaO2／FiO2下降，PaCO2、W／D和LC升高，血清TXB2、6-keto PGF1α浓度和TXB2／6-keto PGF1α比值升高，血清TNF—α、IL1β和IL-6浓度升高，FA组W／D和Lc升高，血清TXB2和6-keto PGF1α浓度升高，血清TNF-α、IL-1β、IL-6和IL-10浓度升高（P〈0．05或0．01）。与LPS组比较，FA组PaCO2、W／D和LC降低，PaO2和PaO2／FiO2升高，血清TXB2、6-keto PGF1α浓度和TXB2／6-keto PGF1α比值降低，血清IL-1β、IL-6和TNF—α浓度降低，血清IL-10浓度升高（P〈0．05）。FA组肺组织病理学损伤较LPS组轻。结论氟比洛芬酯预先给药可减轻内毒素性急性肺损伤，可能与维持血液TXA2和PGI2平衡及抑制炎性反应有关。
Objective To investigate the effect of flurbiprofen axetil （FA） on the acute lung injury （ALI） induced by LPS in rats. Methods Forty male SD rats weighing 190-220 g were randomly divided into 3 groups： group Ⅰ control （C, n=8）; group Ⅱ LPS（n = 16） and group Ⅲ FA＋LPS（n= 16）. In group Ⅱ and Ⅲ LPS 5 mg/kg in 1 ml of normal saline （NS） was given iv. In group Ⅲ FA 6 mg/kg in NS 1 ml was given Ⅳ 0.5 h before LPS administration. In group Ⅱ and Ⅲ 8 animals were killed at 2 h （T1） and 4 h （T2） after LPS administration respectively. Blood samples were obtained at T1 and T2 for blood gas analysis and determination of serum TXB2, 6-keto PGF1α （by radio-immuno assay）, TNF-α, IL-1β, IL-6 and IL-10 concentrations （by ELISA）. Lungs were removed for determination of W/D lung weight ratio, lung water content （LC） and microscopic examination. Results Compared with group C, LPS significantly decreased PaO2, PaO2/FiO2 and increased PaCO2, W/D lung weight ratio, LC, serum TXB2, 6-keto-PGF1α concentrations, TXB2/6-keto-PGF1α ratio and serum IL-1β, TNF-α, and IL-6 concentrations in LPS group. Pulmonary edema and hemorrhage were observed in LPS group. FA pretreatment significantly attenuated LPS-induced blood gas, bio-chemical and pulmonary histological changes in group m Conclusion Flurbiprofen axetil pretreatment can protect the lungs against LPS- induced acute injury by down-regulating TXB2/6-keto-PGF1α ratio and inhibiting inflammatory response.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2008年第12期1117-1119,共3页
Chinese Journal of Anesthesiology
