摘要
目的检测慢性牙周炎患者和牙周健康者龈下菌斑中牙龈卟啉单胞菌(P.gingivalis)PG0717基因,探讨PG0717基因与牙周临床指数之间的关系。方法选取慢性牙周炎(CP)患者90例和牙周健康者90例,共采集龈下菌斑标本540个;记录临床牙周指数(牙周探诊深度、临床附着丧失和探诊出血);设计特异性引物检测P.gingivalis阳性龈下菌斑标本的PG0717基因。结果在P.gingivalis阳性龈下菌斑中,CP组PG0717基因检出率显著高于对照组,分别为56.22%和41.27%(χ2=4.50,P<0.05);随着牙周探诊深度、临床附着丧失加重和探诊出血趋势的增加,CP组该基因检出率呈现增高趋势。结论PG0717基因与P.gingivalis的致病性有关。
Objective The aim of the present study was to investigate PG0717 gene of Porphyromonas gingivalis （P.gingivalis） in subgingival plaque of the chronic periodontitis patients and periodontal healthy subjects, and to find out the relationship of detection rate of PG0717 and periodontal clinical parameters. Methods A total of 540 subgingival plaque samples were collected from 180 subjects including chronic periodontitis（CP） patients（n=90） and periodontal healthy individuals （n=90）. The periodontal clinical parameters including probing depth （PD）, clinical attachment loss （CAL）, and bleeding on probing（BOP） were estimated by Florida probe. The extracted DNA samples of P.gingivalis positive was amplified with the sequence specific primers designed to obtain the PG0717 gene. Results In subgingival plaque of P.gingivalis positive, the detection rate of PG0717 in CP group was significantly higher than that in periodontal healthy subjects （56.22% versus 41.27%, χ^2=4.50, P〈0.05）. The detection rate of PG0717 in CP group showed the increasing tendency in accordance with the depth of PD and CAL. A higher detection rate of PG0717 was observed in the sites of BOP positive than that in BOP negative（57.73% versus 14.29%, χ^2=42.01, P〈 0.01）. Conclusion The findings suggest that the PG0717 gene may influence the virulence of P.gingivalis.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2008年第6期648-651,共4页
West China Journal of Stomatology
基金
国家自然科学基金资助项目(30772427)
辽宁省教育厅科学技术研究基金资助项目(20061021)
