摘要
[目的]为评价炎毒热清（AEE）及其微乳制剂（AEE-ME）的安全性和抑菌活性。[方法]用灌胃法对小鼠进行急性毒性试验,并采用改良寇氏法计算半数致死量及其95%可信区间;采用杯碟法研究抑菌作用。[结果]AEE经小鼠口服LD50为10.937 g/kg,95%可信区间为9.309-12.850 g/kg;AEE-ME经小鼠口服LD50为5.357 g/kg,95%可信区间为4.388-6.566 g/kg。AEE对猪大肠杆菌O149、金黄色葡萄球菌、鸡白痢沙门氏菌、无乳链球菌的MIC分别为10.00、20.00、20.00、10.00 mg/ml;AEE-ME对上述4种菌的MIC依次为5.00、10.00、5.00、5.00 mg/ml,对绿脓杆菌的MIC为20.00 mg/ml。[结论]AEE属实际无毒性物质,AEE-ME为低毒性物质,二者均有抑菌活性且AEE-ME抑菌作用强于AEE。
[Objective] The aim of this study is to evaluate the safety and bacteriostasis of Yandureqing（AEE） and its microemulsion（AEE-ME）.[Method] The acute toxicity was tested in mice by intragastric administration,and median lethal dose（LD50） as well as its 95% confidence interval was calculated by modified Karber method;the bacteriostasis was investigated by cylinder plate method.[Result] LD50 of AEE in mice was 10.937 g/kg with the 95% confidence interval of 9.309-12.850 g/kg;and LD50 of AEE-ME in mice was 5.357 g/kg with the 95% confidence interval of 4.388-6.566 g/kg.The MICs of AEE to Escherichia coli O149 from swine,Staphylococcus aureus,Salmonella pullorum and Streptococcus agalactiae were 10.00,20.00,20.00 and 10.00 mg/ml,respectively;while the MICs of AEE-ME to the 4 kinds of bacteria mentioned above were 5.00,10.00,5.00 and 5.00 mg/ml in turn,and that to Pseudomonas aeruginosa was 20.00 mg/ml.[Conclusion] AEE is an actually nontoxic drug and AEE-ME belongs to the low toxic preparation.AEE and AEE-ME have obvious bacteriostasis,in which AEE-ME is superior to AEE.
出处
《安徽农业科学》
CAS
北大核心
2008年第36期15912-15914,共3页
Journal of Anhui Agricultural Sciences
基金
中央级公益性科研院所基本科研业务费专项资金项目(BRF060403)
甘肃省支撑计划项目(0804NKCA074)
