摘要
[目的]为进一步开发石斛药用资源提供基础数据。[方法]将人宫颈癌细胞HelaS3和肝癌HepG2细胞用含10%新生小牛血清的RPMI-1640培养基在37℃和5%CO2条件下培养,比较了霍山石斛、铁皮石斛、金钗石斛和马鞭石斛水提物对HelaS3和HepG2细胞的体外抑制作用。[结果]4种石斛水提物对HelaS3细胞和HepG2细胞均有不同程度的抑制作用,且在所选浓度范围内对剂量和时间有依赖性。4种石斛水提物对HelaS3细胞的IC50分别是11.63、17.71、15.11和5.89 mg/ml;对HepG2细胞的IC50分别是10.40、22.95、3.80和17.76 mg/ml。显微观察显示,处理组的活细胞多呈红色,核呈固缩状或圆珠状,有明显的染色质凝集和细胞凋亡特征。[结论]剂量效应分析表明,对HelaS3细胞而言,马鞭石斛水提物抑瘤效果较好;对HepG2细胞而言,金钗石斛水提物抑瘤效果较好。
[Objective] The study was to provide the foundation data for further development of Dendrobium medicinal resources.[Method] HelaS3 cells and HepG2 cells were put on the RPMI-1640 medium with 10% new newborn calf serum and cultured under 37 ℃ and 5% CO2 to compare in vitro inhibitory effects of water extracts from Dendrobium huoshanense,D.candidum,D.nobile and D.fimbriatum on HelaS3 cells and HepG2 cells.[Result] The water extracts from 4 species of Dendrobium could inhibit the growth of HelaS3 cells and HepG2 cells to different extent,with the inhibitory effects being dose-dependent and time-dependent in the tested concn.range.The IC50 of 4 species of Dendrobium on HelaS3 cells were 11.63,17.71,15.11 and 5.89 mg/ml,resp.and that on on HepG2 cells were 10.40,22.95,3.80 and 17.76 mg/ml,resp.The microscope observations showed that most of the living cells treated with extracts appeared red,with pycnosis of nucleu,agglutination of chromatin and occurrence of apoptosis induced by extracts.[Conclusion] The dose effect analysis showed that the tumor inhibiting effect of water extracts from D.fimbriatum on HelaS3 cells was better and that from D.nobile HepG2 was better.
出处
《安徽农业科学》
CAS
北大核心
2008年第36期15968-15970,共3页
Journal of Anhui Agricultural Sciences
基金
合肥工业大学青年基金(073001F)
合肥工业大学大学生创新基金(XS07046)
关键词
霍山石斛
铁皮石斛
金钗石斛
马鞭石斛
肿瘤细胞
抑制作用
Dendrobium huoshanense
D.candidum
D.nobile
D.fimbriatum
Tumor cells
Inhibitory effects
