摘要
目的:测定痛安注射液(青风藤,白屈菜等)中青藤碱和白屈菜碱的含量。方法:采用HPLC法,以甲醇-0.1%三乙胺为流动相,线性梯度洗脱,检测波长:280nm。结果:青藤碱在162μg～1620μg范围内呈良好的线性关系,白屈菜碱在35μg～350μg范围内呈良好的线性关系。青藤碱的平均回收率为99.56%,RSD为0.41%(n=5)。白屈菜碱的平均回收率为99.46%,RSD为0.62%(n=5)。结论:本试验方法简便,重现性好,专属性强,可用于痛安注射液的质量控制。
AIM ： To determine the contents Of sinomenine and chelidonine in Tong＇ an Injection （ Caulis sino- menii, Chelidonium majus Linn, etc）. METHODS： HPLC was used. The conditions included the gradient elution with methanol-0. 1% triethylamine. The detection wavelength was set at 280 nm. RESULTS： The calibration curve was linear in the range of 162-1 620 μg for the sinomenine and the range of 35-350 μg for ehelidonine, re- spectively. The average recovery for sinomenine was 99.56% and the relative standard deviation was 0.41% （n = 5 ）. The average recovery for chelidonine was 99.46% and the relative standard deviation was 0.62% （ n = 5 ）. CONCLUSION： The method is simple, rapid and specialty. It can be used for the determination of sinomenine and chelidonine in Tong＇an Injection.
出处
《中成药》
CAS
CSCD
北大核心
2009年第2期235-237,共3页
Chinese Traditional Patent Medicine
