摘要
背景:随着胰腺移植供体的不足,胰段移植已倍受关注。从解剖学角度认识胰腺各段胰岛和胰岛细胞(A,B细胞)的分布规律,可否得出胰段移植的最佳选择。目的:观察幼年大鼠胰腺胰尾的微细结构,胎尸胰腺胰尾的微细结构,胰岛B细胞胰高血糖素和胰岛素阳性表达面积及胰岛B细胞免疫阳性染色面积,并予以比较。设计、时间及地点:对比观察,于2006-01/2007-06在福建医科大学人体解剖组织胚胎学系实验室完成。材料:出生1周内健康SD大鼠33只,3～10个月龄胎尸胰腺80个,每月龄10个。胰高血糖素抗血清为武汉博士德生物工程公司产品,胰岛素抗血清为北京中山金桥生物技术公司产品,图像处理系统(Videopro32型)由福建医科大学人体解剖实验室提供,Hu-12A型透射电镜为日本电子株式会社产品。方法:随机选择30只大鼠,取胰尾切片苏木精-伊红染色后光镜观察,显微测微器测量胰岛个数和面积,用胰高血糖素抗血清和胰岛素抗血清进行免疫组织化学染色。另3只大鼠取胰尾组织块铀铅染色,每月龄胎尸各取2例胰尾组织,1例行ABC法胰岛素免疫组化染色,另1例行铀铅染色。主要观察指标:透射电镜观察并比较胎尸胰及幼年大鼠胰腺B细胞及胰岛形态和数量、结构及其分泌颗粒。结果:幼年大鼠胰尾组织中胰岛B细胞内分泌颗粒多,内质网、高尔基复合体和线粒体等细胞器发达,胰岛数目分布多,胰岛B细胞的免疫阳性染色面积大。胎尸胰尾有较多而密的胰岛B细胞散在分布,胰岛B细胞分泌颗粒多。结论:幼年大鼠和胎尸胰腺细胞的分泌活动均旺盛的,消化功能强,适宜作为胰腺胰段胰岛移植供体,且以胰尾为最佳。
BACKGROUND： Pancreatic segmental transplantation attracts more attention when donor is insufficient. The distribution of pancreatic islet and pancreatic islet cells （A, B cells） is investigated by means of anatomy to select the best choice for pancreatic segmental transplantation.
OBJECTIVE： Microstructure, positive staining area of pancreatic islets B cells after glucagon and insulin expression, and immune positive staining area of pancreatic islets B cells were observed in the pancreatic tails of both young rats and fetus cadavers, to provide a necessary reference for fetus pancreatic segmental islets transplantation.
DESIGN, TIME AND SETTING： The comparative observation was performed in the Laboratory of Human Anatomy Staff Room of Fujian Medical University from January 2006 to June 2007.
MATERIALS： A total of 33 healthy SD rats which born within 1 weak were selected, and each 10 cadavers＇ fetuses were selected from pancreas of cadavers of 3 10 months age with a total of 80 cases. The antiserum against glucagon was provided by Boster Biological Technology, Co., Ltd （Wuhan, China）, insulin antiserum was the production of Zhongshan Goldenbridge Biotechnology Co., Ltd. The image Processing System （Videopro32） was supplied by the Laboratory of Human Anatomy Staff Room of Fujian Medical University, and the Hu-12A transmission electron microscope was purchased from Japan Electron Optics Laboratory Co., Ltd.
METHODS： A total of 30 pancreatic tails of rats were randomized selected and observed under light microscope following hematoxylin-eosin staining, measured the number and area of pancreatic islet. Then immunohistochemistry staining was carried out using pancreatic glucago antiserum and insulin antiserum. The pancreatic tails of other 3 rats were underwent uranium lead staining. 2 tails of pancreas were took out from each age cadavers, 1 case was studied with immunohistochemical ABC method and the other case was stained by uranium lead.
MAIN OUTCOME MEASURES： Cellular changes, quantity, secretory response, and morphological details of pancreatic B-cells and pancreatic islets were observed by transmission electron microscope.
RESULTS： There were abundant of secretory granules within islets B cells in young rats cauda pancreatitis, and organelles such as the endoplasmic reticulum, the Golgi complex and the mitochondria were flourished with greater immune positive areas. In addition, there were more secretory granules in the pancreatic islets B cells which distributed in the fetus pancreatic tails with great number and density.
CONCLUSION： Both young rats and fetus cadavers with vigorous secretory activity and digestive function are capable for pancreatic segmental transplantation, especially those of cauda pancreatis.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2009年第5期865-868,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
福建省教育基金项目(JB06157)
莆田市科学技术局基金项目(2005S08)
莆田学院基金项目(2005057)~~
