摘要
利用阿魏酸酯酶,水解天然木质纤维素原料中半纤维素与木质素之间的阿魏酸酯键,从破坏两者共价键连接的角度,探索阿魏酸酯酶促进纤维素酶水解汽爆稻草中纤维素的可行性。结果显示,当阿魏酸酯酶加入量为240mu/g底物、水解72h时,汽爆稻草纤维素的酶解率、不溶性底物失重率较不加阿魏酸酯酶分别增加了32.00%、32.77%;阿魏酸酯酶(300mu/g底物)作用120min后,纤维素酶对汽爆稻草纤维素的酶解率、不溶性底物失重率分别增加了29.85%、32.48%。通过比较不同酶法处理后的汽爆稻草的可及度和红外光谱图发现,阿魏酸酯酶能有效地水解原料中的酯键,提高原料可及度50%以上。由此表明,阿魏酸酯酶和纤维素酶之间存在较大的协同作用,添加阿魏酸酯酶能够提高纤维素酶对天然木质纤维素的酶解效率。
Ferulic acid esterase （FAE） was used to hydrolyze feruloyl ester linkages between hemicellulose and lignin in natural lignocellulose, and the possibility of FAE accelerating cellulase to hydrolyze steam-exploded rice straw by breaking covalent linkages was studied. When the dosage of FAE was 240 mu/g substrate, the cellulose conversion rate and the weight-loss rate of insoluble substrate at 72 h were respectively 32.00% and 32.77%, more than without using FAE; Cellulose conversion rate and the weight-loss rate of insoluble substrate were respectively 29.85% and 32.48% with FAE （300 mu/g substrate） processing time of 120 min. By comparison of the accessibility and FT-IR spectra of lignocellulosic material treated by different enzyme methods, it indicated that FAE hydrolyzed some ester bonds within it, and improved the accessibility by over 50%. It is concluded that FAE and cellulase have great synergistic effect, and FAE can help cellulase hydrolyze natural lignocellulose and enhance hydrolytic efficiency.
出处
《生物工程学报》
CAS
CSCD
北大核心
2009年第1期49-54,共6页
Chinese Journal of Biotechnology
基金
国家科技支撑计划项目(No.2007BAD39B01)资助~~
关键词
阿魏酸酯酶
纤维素酶
汽爆稻草
协同作用
ferulic acid esterase, cellulose, steam-exploded rice straw, synergistic effect
